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CLCN3 Antibody (MA5-45555) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with Clcn3 monoclonal antibody (Product # MA5-45555) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min RT, 5 min RT. Localization: Membrane, Endosome, Endosome membrane, Cytoplasmic Vesicle, Secretory Vesicle Membrane. Magnification:... View More
产品信息
MA5-45555
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
N258/5
抗原
Synthetic peptide amino acids 98-115 (cytoplasmic N-terminus) of rat Clcn3
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
9.1mM sodium bicarbonate/PBS, pH 7.4, with 640.91mM DMSO, 136.36mM ethanolamine
内含物
no preservative
保存条件
4° C, store in dark
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2932009
产品详细信息
1 µg/mL of MA5-45555 was sufficient for detection of Clcn3 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 90kDa. Does not cross-react with Clcn4 or Clcn5 (based on KO validation results).
This antibody was formerly sold as clone S258-5.
靶标信息
Mediates the exchange of chloride ions against protons. Functions as antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. May play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could help neuronal cells to establish short-term memory.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: chloride channel 3; Chloride channel protein 3; chloride channel, voltage-sensitive 3; Chloride transporter ClC-3; CIC3; ClC-3; CLCN 3; DKFZp564I0463; H(+)/Cl(-) exchange transporter 3; protein kinase C-regulated chloride channel
基因别名: ClC-3; CLC3; CLCN3
UniProt ID: (Human) P51790, (Rat) P51792, (Mouse) P51791
Entrez Gene ID: (Human) 1182, (Rat) 84360, (Mouse) 12725
voltage-gated chloride channel activity
chloride channel activity
protein binding
ATP binding
antiporter activity
PDZ domain binding
chloride ion binding
protein homodimerization activity
protein heterodimerization activity
volume-sensitive chloride channel activity
ion channel activity
voltage-gated ion channel activity
nucleotide binding
ion channel
transport
regulation of pH
negative regulation of cell volume
endosomal lumen acidification
chloride transmembrane transport
regulation of anion transmembrane transport
ion transport
chloride transport
phagocytosis, engulfment
adult locomotory behavior
ion transmembrane transport
regulation of ion transmembrane transport
synaptic transmission, glutamatergic
regulation of anion transport
photoreceptor cell maintenance
synaptic transmission, GABAergic
neuron cellular homeostasis
synaptic vesicle lumen acidification
positive regulation of reactive oxygen species biosynthetic process
transmembrane transport
