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CLCNKB Antibody (PA5-97592) in ICC/IF
Immunofluorescent analysis of CLCNKB in HepG2 cells using a CLCNKB polyclonal antibody (Product # PA5-97592) at a dilution of 1:100. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.
产品信息
PA5-97592
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant Human Chloride channel protein ClC-Kb protein (538-687AA)
偶联物
形式
Liquid
浓度
2.96 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.03% ProClin 300
保存条件
-20°C or -80°C if preferred
运输条件
Wet ice
RRID
AB_2812207
靶标信息
Chloride channel Kb (CLCNKB) is a member of the CLC family of voltage-gated chloride channels, which comprises at least 9 mammalian chloride channels. Each is believed to have 12 transmembrane domains and intracellular N and C termini. Mutations in CLCNKB result in the autosomal recessive Type III Bartter Syndrome. CLCNKA and CLCNKB are closely related (94% sequence identity), tightly linked (separated by 11 kb of genomic sequence) and are both expressed in mammalian kidney.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: chloride channel K2; Chloride channel Kb; Chloride channel protein ClC-Kb; chloride channel, kidney, B; chloride channel, voltage-sensitive Kb; ClC-K2; CLCNK; hClC-Kb; MGC24087; RP11-5P18.8
基因别名: ClC-K2; ClC-K2L; ClC-Kb; Clck2; CLCKB; Clcnk1l; CLCNKB
UniProt ID: (Human) P51801, (Rat) P51802
Entrez Gene ID: (Human) 1188, (Rat) 79430
