Invitrogen

CLIC2 Monoclonal Antibody (A5G9)

CLIC2 Antibody in Immunocytochemistry (ICC/IF)

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CLIC2 Antibody (MA5-42553) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of of CLIC2 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with CLIC2 Monoclonal Antibody (Product # MA5-42553) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (... View More

CLIC2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

MA5-42553

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:100-1:500

免疫细胞化学 (ICC/IF)

1:50-1:100

流式细胞分析 (Flow)

1:100-1:500

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

A5G9

抗原

Recombinant protein within human CLIC2 aa 50-247/247

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2 mg/mL

纯化类型

Protein G

保存液

TBS, pH 7.4, with 50% glycerol, 0.2% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2911694

产品详细信息

Positive Control: K562 cell lysates, human liver tissue lysate, human skeletal muscle tissue lysate, HepG2, human fetal skeletal muscle tissue, human liver tissue, SH-SY5Y.

Subcellular Location: Cytoplasm, Membrane.

靶标信息

Chloride intracellular channel 2 is a member of the p64 family, a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. CLIon Channel 2 encodes a protein that is detected in fetal liver and adult skeletal muscle tissue. CLIon Channel2 maps to the candidate region on chromosome X for incontinentia pigmenti.

仅用于科研。不用于诊断过程。未经明确授权不得转售。