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CLIC4 Antibody (ACL-024-200UL) in ICC/IF
Expression of CLIC4 in human pancreatic carcinoma PANC-1 cells - Immunocytochemical staining of fixed and permeabilized human pancreatic carcinoma PANC-1 cells. A. Cells were stained with Anti-CLIC4 Antibody (#ACL-024), (1:200), followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody (red). B.Cell nuclei were visualized using Hoechst 33342 (blue).C. Live image of the cells.
Product Details
ACL-024-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)NGLKEEDKEPLIE, corresponding to amino acid residues 8-20 of human CLIC4, Intracellular, N-terminus
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: chloride intracellular channel 4 (mitochondrial); chloride intracellular channel 4 like; Chloride intracellular channel protein 4; DKFZP566G223; FLJ38640; Glutaredoxin-like oxidoreductase CLIC4; Intracellular chloride ion channel protein p64H1; mc3s5/mtCLIC; mitochondrial chloride intracellular channel 4
Gene Aliases: CLIC4; CLIC4L; D0Jmb3; H1; huH1; mc3s5; MTCLIC; p64H1; TU-74
UniProt ID: (Human) Q9Y696, (Mouse) Q9QYB1, (Rat) Q9Z0W7
Entrez Gene ID: (Human) 25932, (Mouse) 29876, (Rat) 83718
angiogenesis
endothelial cell morphogenesis
glutathione metabolic process
chloride transport
apoptotic process
vacuolar acidification
fertilization
cell differentiation
keratinocyte differentiation
negative regulation of cell migration
regulation of ion transmembrane transport
establishment or maintenance of apical/basal cell polarity
multicellular organism growth
branching morphogenesis of an epithelial tube
regulation of cytoskeleton organization
retina vasculature morphogenesis in camera-type eye
cellular response to calcium ion
chloride transmembrane transport
transport
ion transport
Performance Guarantee
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