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CLIC4 Antibody (PA5-28146) in WB
Western Blot analysis of CLIC4 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a CLIC4 Polyclonal Antibody (Product # PA5-28146) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Product Details
PA5-28146
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 3 and 253 of Human CLIC4
Conjugate
Form
Liquid
Concentration
0.83 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2545622
Product Specific Information
Recommended positive controls: HeLa, Raji.
Predicted reactivity: Mouse (98%), Rat (96%), Zebrafish (85%), Xenopus laevis (87%), Rhesus Monkey (99%), Bovine (98%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells).
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: chloride intracellular channel 4 like; Chloride intracellular channel protein 4; DKFZP566G223; FLJ38640; Glutaredoxin-like oxidoreductase CLIC4; Intracellular chloride ion channel protein p64H1
Gene Aliases: CLIC4; CLIC4L; H1; huH1; MTCLIC; p64H1
UniProt ID: (Human) Q9Y696
Entrez Gene ID: (Human) 25932
angiogenesis
endothelial cell morphogenesis
glutathione metabolic process
chloride transport
apoptotic process
vacuolar acidification
fertilization
cell differentiation
keratinocyte differentiation
negative regulation of cell migration
regulation of ion transmembrane transport
establishment or maintenance of apical/basal cell polarity
multicellular organism growth
branching morphogenesis of an epithelial tube
regulation of cytoskeleton organization
retina vasculature morphogenesis in camera-type eye
cellular response to calcium ion
chloride transmembrane transport
Performance Guarantee
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