Invitrogen

CLPX Polyclonal Antibody

CLPX Antibody in Immunocytochemistry (ICC/IF)

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CLPX Antibody (PA5-79052) in ICC/IF

Immunocytochemistry analysis of CLPX using anti-CLPX antibody (Product # PA5-79052) . CLPX was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-CLPX antibody (Product # PA5-79052) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visua... View More

CLPX Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-79052

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫组化（冰冻） (IHC (F))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived human CLPX recombinant protein (Position: A337-E574).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2746168

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of clpP.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

CLPP Depletion Causes Diplotene Arrest; Underlying Testis Mitochondrial Dysfunction Occurs with Accumulation of Perrault Proteins ERAL1, PEO1, and HARS2.

Cells

Key J,Gispert S,Koornneef L,Sleddens-Linkels E,Kohli A,Torres-Odio S,Koepf G,Amr S,Reichlmeir M,Harter PN,West AP,Münch C,Baarends WM,Auburger G

PA5-79052 was used in Western Blotting to propose that the loss of CLPP leads to the extrusion of mitochondrial nucleotide-binding proteins to cytosol and nucleus, affecting late meiotic prophase progression, and causing cell death prior to M-phase entry.

Thu Dec 22 00:00:00 UTC 2022

Inactivity of Peptidase ClpP Causes Primary Accumulation of Mitochondrial Disaggregase ClpX with Its Interacting Nucleoid Proteins, and of mtDNA.

Cells

Key J,Torres-Odio S,Bach NC,Gispert S,Koepf G,Reichlmeir M,West AP,Prokisch H,Freisinger P,Newman WG,Shalev S,Sieber SA,Wittig I,Auburger G

PA5-79052 was used in Western Blot to study global proteome profiles to define mitochondrial matrix peptidase ClpP substrates among the substrate-selecting AAA+ ATPase ClpX interactors.

Mon Nov 29 00:00:00 UTC 2021

Inactivity of Peptidase ClpP Causes Primary Accumulation of Mitochondrial Disaggregase ClpX with Its Interacting Nucleoid Proteins, and of mtDNA.

Cells

Key J,Torres-Odio S,Bach NC,Gispert S,Koepf G,Reichlmeir M,West AP,Prokisch H,Freisinger P,Newman WG,Shalev S,Sieber SA,Wittig I,Auburger G

PA5-79052 was used in Western Blot to study global proteome profiles to define mitochondrial matrix peptidase ClpP substrates among the substrate-selecting AAA+ ATPase ClpX interactors.

Mon Nov 29 00:00:00 UTC 2021

生物信息学

蛋白别名: ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial; caseinolytic peptidase X; caseinolytic protease X; ClpX caseinolytic peptidase X homolog; ClpX caseinolytic protease X homolog; energy-dependent regulator of proteolysis

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基因别名: AU014732; CLPX; E330029I21

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UniProt ID: (Human) O76031, (Mouse) Q9JHS4, (Rat) Q5U2U0

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Entrez Gene ID: (Human) 10845, (Mouse) 270166, (Rat) 300786

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功能