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CNOT7 Antibody (PA5-101270) in ICC/IF
Immunocytochemistry analysis of CNOT7 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with CNOT7 polyclonal antibody (Product # PA5-101270) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-101270
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human CNOT7(Accession Q9UIV1), corresponding to amino acid residues E243-S285.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2850709
产品详细信息
Antibody detects endogenous levels of total CNOT7.
靶标信息
CNOT7 (CCR4-NOT transcription complex subunit 7) has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT8. CNOT7 is a catalytic component of the CCR4-NOT complex, which is one of the major cellular mRNA deadenylases, and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation, and general transcription regulation. During miRNA-mediated repression, the complex seems to act as a transcriptional repressor during translational initiation, as well. Additional complex functions may be a consequence of its influence on mRNA expression. CNOT7 associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti-proliferative activity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: BTG1-binding factor 1; CAF-1; Caf1a; carbon catabolite repressor protein (CCR4)-associative factor 1; catabolite repressor protein (CCR4)-associative factor 1; CCR4-associated factor 1; CCR4-NOT transcription complex subunit 7; POP2 homolog
基因别名: AU022737; CAF-1; CAF1; Caf1a; CNOT7; hCAF-1; Pop2
UniProt ID: (Human) Q9UIV1, (Mouse) Q60809
Entrez Gene ID: (Human) 29883, (Mouse) 18983
3'-5'-exoribonuclease activity
transcription factor activity, sequence-specific DNA binding
RNA binding
exoribonuclease activity
poly(A)-specific ribonuclease activity
signal transducer activity
protein binding
metal ion binding
nucleic acid binding
nuclease activity
exonuclease activity
hydrolase activity
mRNA polyadenylation factor
nuclear-transcribed mRNA poly(A) tail shortening
deadenylation-dependent decapping of nuclear-transcribed mRNA
carbohydrate metabolic process
transcription, DNA-templated
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
signal transduction
positive regulation of cell proliferation
negative regulation of cell proliferation
negative regulation of gene expression
gene silencing by RNA
cytoplasmic mRNA processing body assembly
gene silencing by miRNA
exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay
positive regulation of transcription from RNA polymerase II promoter
positive regulation of nuclear-transcribed mRNA poly(A) tail shortening
positive regulation of mRNA catabolic process
RNA phosphodiester bond hydrolysis, exonucleolytic
positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
regulation of transcription, DNA-templated
regulation of translation
