Invitrogen

CNTF Polyclonal Antibody, Biotin, PeproTech®

图: 1 / 3

CNTF Antibody (500-P79BT-25UG) in WB

Western Blot: To detect Rat CNTF by Western Blot analysis CNTF Polyclonal Antibody, Biotin (Product # 500-P79BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat CNTF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Product Image Gallery

产品信息

500-P79BT-25UG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

酶联免疫吸附实验 (ELISA)

0.25-1.0 µg/mL

产品规格

种属反应

Rat

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived Recombinant Rat CNTF

查看免疫原

偶联物

Biotin Biotin Biotin

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

AB_2930771

产品详细信息

AA Sequence of recombinant protein: AFAEQTPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNINLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LMLQVSAFAY QLEELMVLLE QKIPENEADG MPATVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HQMGISALES HYGAKDKQM.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat CNTF. Anti-Rat CNTF-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Rat CNTF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat CNTF (500-P79) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat CNTF.

Western Blot: To detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat CNTF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P79BT-1MG will be provided as 2 x 500 µg

靶标信息

The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of ZFP91 and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse.

仅用于科研。不用于诊断过程。未经明确授权不得转售。