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CNTF Polyclonal Antibody

CNTF Antibody in Immunocytochemistry (ICC/IF)

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CNTF Antibody (ANT-031-200UL) in ICC/IF

Expression of CNTF in rat C6 glioma cells - Immunocytochemical staining of fixed and permeabilized rat C6 glioma cells. A. Cells were stained with Anti-CNTF Antibody (#ANT-031), (1:200), followed by goat Anti-rabbit-DyLight-594 secondary Antibody (red). B. Cell nuclei were visualized with the membrane-permeable DNA dye Hoechst 33342 (blue staining). C. Live view of the cells.

CNTF Antibody in Immunohistochemistry (IHC)

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产品信息

ANT-031-200UL

应用

建议稀释比

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免疫印迹 (WB)

1:200

免疫组化 (IHC)

1:200

免疫细胞化学 (ICC/IF)

1:200

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

(C)ELSQWTVRSIHDLR, corresponding to amino acid residues 164-177 of human CNTF, C-terminus

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.8 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 1% BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Reconstitution: 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

靶标信息

The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of ZFP91 and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse.

仅用于科研。不用于诊断过程。未经明确授权不得转售。