Invitrogen

COL1A1 Polyclonal Antibody

COL1A1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 21

COL1A1 Antibody (PA5-29569) in ICC/IF

Immunofluorescence analysis of COL1A1 was performed using 70% confluent log phase SK-N-SH and HT-29 cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 5 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with COL1A1 Polyclonal Antibody (Product # PA5-29569) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (P... View More

COL1A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

COL1A1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

产品信息

PA5-29569

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

查看11篇已发表文章 11篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

查看3篇已发表文章 3篇已发表文章

免疫沉淀 (IP)

1:100-1:500

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Chicken, Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 219 and 478 of Human COL1A1

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.09 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 1% BSA, 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2547045

产品详细信息

Recommended positive controls: SK-N-SH, SK-N-AS, NIH3T3.

Predicted reactivity: Mouse (96%), Rat (94%), Xenopus laevis (83%), Dog (98%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

The protein encoded by this gene is the pro-alpha1 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Obesity Contributes to Transformation of Myometrial Stem-Cell Niche to Leiomyoma via Inducing Oxidative Stress, DNA Damage, Proliferation, and Extracellular Matrix Deposition.

Genes

Afrin S,Kirschen GW,Borahay MA

PA5-29569 was used in Western Blot to determine how adipocytes and adipocyte-secreted factors affect changes in myometrial stem cells (MSCs) in a manner that promotes the growth of uterine leiomyomas.

Tue Aug 15 00:00:00 UTC 2023

Kaempferide Prevents Photoaging of Ultraviolet-B Irradiated NIH-3T3 Cells and Mouse Skin via Regulating the Reactive Oxygen Species-Mediated Signalings.

Antioxidants (Basel, Switzerland)

Choi JK,Kwon OY,Lee SH

Published figure using COL1A1 polyclonal antibody (Product # PA5-29569) in Western Blot

Wed Dec 21 00:00:00 UTC 2022

Leptin induces leiomyoma cell proliferation and extracellular matrix deposition via JAK2/STAT3 and MAPK/ERK pathways.

F&S science

Reschke L,Afrin S,El Sabah M,Charewycz N,Miyashita-Ishiwata M,Borahay MA

PA5-29569 was used in Western Blot to suggest that leptin, acting through the JAK2/STAT3 and MAPK/ERK pathways, is involved in the development of uterine leiomyomas, which may partly explain their increased incidence in obese women.

Tue Nov 01 00:00:00 UTC 2022

Cardioids reveal self-organizing principles of human cardiogenesis.

Cell

Hofbauer P,Jahnel SM,Papai N,Giesshammer M,Deyett A,Schmidt C,Penc M,Tavernini K,Grdseloff N,Meledeth C,Ginistrelli LC,Ctortecka C,Šalic Š,Novatchkova M,Mendjan S

PA5-29569 was used in Immunocytochemistry to developmental heart chamber defects.

Thu Jun 10 00:00:00 UTC 2021

Impaired non-canonical transforming growth factor-β signalling prevents profibrotic phenotypes in cultured peptidylarginine deiminase 4-deficient murine cardiac fibroblasts.

Journal of cellular and molecular medicine

Akboua H,Eghbalzadeh K,Keser U,Wahlers T,Paunel-Görgülü A

PA5-29569 was used in Western Blotting to investigate the impact of peptidylarginine deiminase 4 on cardiac fibroblast function and myofibroblast transdifferentiation in vitro.

Fri Oct 01 00:00:00 UTC 2021

Impaired non-canonical transforming growth factor-β signalling prevents profibrotic phenotypes in cultured peptidylarginine deiminase 4-deficient murine cardiac fibroblasts.

Journal of cellular and molecular medicine

Akboua H,Eghbalzadeh K,Keser U,Wahlers T,Paunel-Görgülü A

PA5-29569 was used in Western Blotting to investigate the impact of peptidylarginine deiminase 4 on cardiac fibroblast function and myofibroblast transdifferentiation in vitro.

Fri Oct 01 00:00:00 UTC 2021

Translation of Tudor-SN, a novel terminal oligo-pyrimidine (TOP) mRNA, is regulated by the mTORC1 pathway in cardiomyocytes.

RNA biology

Gan S,Su C,Ma J,Liu M,Cui X,Xin L,Ren Y,Gao X,Ge L,Wei M,Yang J

PA5-29569 was used in Western Blot to suggest that the translational efficiency of Tudor-SN is regulated by the mTORC1 pathway in myocardia and that Tudor-SN is involved in mTORC1-mediated regulation of cardiomyocytic proliferation and cardiac development.

Tue Jun 01 00:00:00 UTC 2021

An Injectable Fibrin Scaffold Rich in Growth Factors for Skin Repair.

BioMed research international

Shao Z,Lyu C,Teng L,Xie X,Sun J,Zou D,Lu J

PA5-29569 was used in Immunohistochemistry to introduce some characteristics of IFS.

Tue May 25 00:00:00 UTC 2021

Dystrophin Deficiency Causes Progressive Depletion of Cardiovascular Progenitor Cells in the Heart.

International journal of molecular sciences

Jelinkova S,Sleiman Y,Fojtík P,Aimond F,Finan A,Hugon G,Scheuermann V,Beckerová D,Cazorla O,Vincenti M,Amedro P,Richard S,Jaros J,Dvorak P,Lacampagne A,Carnac G,Rotrekl V,Meli AC

PA5-29569 was used in Western Blot to identify the early recruitment of CVPCs in dystrophic hearts and their fast depletion with ageing. This latter depletion may participate in the fibrosis development and the acceleration onset of the cardiomyopathy.

Mon May 10 00:00:00 UTC 2021

Dystrophin Deficiency Causes Progressive Depletion of Cardiovascular Progenitor Cells in the Heart.

International journal of molecular sciences

Jelinkova S,Sleiman Y,Fojtík P,Aimond F,Finan A,Hugon G,Scheuermann V,Beckerová D,Cazorla O,Vincenti M,Amedro P,Richard S,Jaros J,Dvorak P,Lacampagne A,Carnac G,Rotrekl V,Meli AC

Mon May 10 00:00:00 UTC 2021

Deficient Endoplasmic Reticulum Acetyl-CoA Import in Pancreatic Acinar Cells Leads to Chronic Pancreatitis.

Cellular and molecular gastroenterology and hepatology

Cooley MM,Thomas DDH,Deans K,Peng Y,Lugea A,Pandol SJ,Puglielli L,Groblewski GE

PA5-29569 was used in Immunocytochemistry to examine the role of the ER acetyl-CoA transporter AT-1 on pancreatic homeostasis.

Mon Dec 20 00:00:00 UTC 2021

Human Mesenchymal Stromal Cell-Derived Exosomes Promote In Vitro Wound Healing by Modulating the Biological Properties of Skin Keratinocytes and Fibroblasts and Stimulating Angiogenesis.

International journal of molecular sciences

Tutuianu R,Rosca AM,Iacomi DM,Simionescu M,Titorencu I

PA5-29569 was used in Western Blotting and Immunocytochemistry to test if MSC-derived exosomes could accelerate wound healing by enhancing the biological properties of the main cell types involved in the key phases of this process.

Wed Jun 09 00:00:00 UTC 2021

Human Mesenchymal Stromal Cell-Derived Exosomes Promote In Vitro Wound Healing by Modulating the Biological Properties of Skin Keratinocytes and Fibroblasts and Stimulating Angiogenesis.

International journal of molecular sciences

Tutuianu R,Rosca AM,Iacomi DM,Simionescu M,Titorencu I

Wed Jun 09 00:00:00 UTC 2021

Small molecule inhibitor of HSP47 prevents pro-fibrotic mechanisms of fibroblasts in vitro.

Biochemical and biophysical research communications

Miyamura T,Sakamoto N,Kakugawa T,Taniguchi H,Akiyama Y,Okuno D,Moriyama S,Hara A,Kido T,Ishimoto H,Yamaguchi H,Miyazaki T,Obase Y,Ishimatsu Y,Tanaka Y,Mukae H

PA5-29569 was used in Western Blot to demonstrate that HSP47 is a potential therapeutic target for pulmonary fibrosis.

Thu Sep 24 00:00:00 UTC 2020

FOXM1 is a critical driver of lung fibroblast activation and fibrogenesis.

The Journal of clinical investigation

Penke LR,Speth JM,Dommeti VL,White ES,Bergin IL,Peters-Golden M

PA5-29569 was used in Western Blotting to identify FOXM1 as a driver of lung fibroblast activation and underscore the therapeutic potential of targeting FOXM1 for pulmonary fibrosis.

Fri Jun 01 00:00:00 UTC 2018

MiR-132 regulates osteogenic differentiation via downregulating Sirtuin1 in a peroxisome proliferator-activated receptor β/δ-dependent manner.

Biochemical and biophysical research communications

Gong K,Qu B,Liao D,Liu D,Wang C,Zhou J,Pan X

PA5-29569 was used in western blot to elucidate mechanisms by which miR-132 regulates osteogenic differentiation

Fri Sep 09 00:00:00 UTC 2016

Sirtuin1 promotes osteogenic differentiation through downregulation of peroxisome proliferator-activated receptor γ in MC3T3-E1 cells.

Biochemical and biophysical research communications

Qu B,Ma Y,Yan M,Gong K,Liang F,Deng S,Jiang K,Ma Z,Pan X

PA5-29569 was used in western blot to investigate the molecular mechanism of Sirt1 in osteogenic differentiation

Fri Sep 09 00:00:00 UTC 2016

生物信息学

蛋白别名: alpha-1 type 1 collagen; Alpha-1 type I collagen; alpha1(I) procollagen; COL1A; Collagen 1; collagen alpha 1 chain type I; Collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; Collagen I; collagen of skin, tendon and bone, alpha-1 chain; collagen, type 1, alpha 1; collagen, type I, alpha 1; Collagen1A1; pro-alpha-1 collagen type 1; procollagen type I, alpha 1; procollagen, type 1, alpha 1; procollagen, type I, alpha 1; Type I Collagen; type I proalpha 1; type I procollagen alpha 1 chain; type I-alpha 1 collagen

查看更多收起

基因别名: Col1a-1; COL1A1; Cola-1; Cola1; COLIA1; EDSC; Mov-13; Mov13; OI1; OI2; OI3; OI4

查看更多收起

UniProt ID: (Human) P02452, (Rat) P02454, (Mouse) P11087

查看更多收起

Entrez Gene ID: (Human) 1277, (Rat) 29393, (Mouse) 12842

查看更多收起

功能

extracellular matrix structural constituent protein binding identical protein binding metal ion binding platelet-derived growth factor binding molecular_function extracellular matrix structural protein

参与通路