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COL6A1 Antibody (PA5-29068) in ICC/IF
Immunofluorescence analysis of COL6A1 was performed using 70% confluent log phase IMR-90 and SK-O-V3 cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 5 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with COL6A1 Polyclonal Antibody (Product # PA5-29068) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (... View More
产品信息
PA5-29068
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 552 and 826 of Human COL6A1
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
0.1M tris glycine, pH 7, with 10% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2546544
产品详细信息
Recommended positive controls: 293T, U87-MG.
Predicted reactivity: Mouse (85%), Bovine (87%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (typesI, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: alpha 1 (VI) chain (61 AA); Collagen alpha-1(VI) chain; collagen VI, alpha-1 polypeptide; collagen, type VI, alpha 1; procollagen, type VI, alpha 1
基因别名: AI747156; BTHLM1; Col6a-1; COL6A1; OPLL; RGD1565398; UCHMD1
UniProt ID: (Human) P12109, (Mouse) Q04857
Entrez Gene ID: (Human) 1291, (Mouse) 12833, (Rat) 294337
