Invitrogen

COX2 Polyclonal Antibody

COX2 Antibody in Immunocytochemistry (ICC/IF)

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COX2 Antibody (PA1-9032) in ICC/IF

Immunofluorescence analysis of COX2 was performed using 70% confluent log phase RAW 264.7 cells treated with 100 ng/mL of Lipopolysaccharide for 24 Hrs. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with COX2 Polyclonal Antibody (Product # PA1-9032) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Anti... View More

COX2 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

COX2 Antibody in Immunohistochemistry (IHC)

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COX2 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using Anti-COX2 Rabbit Polyclonal Antibody (Product # PA1-9032) shows increased expression of proteins in RAW 264.7 cell line upon LPS treatment. (DOI:10.1038/s41598-017-10720-4). {TM}

产品信息

PA1-9032

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.3 µg/mL

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免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

10 µg/mL

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流式细胞分析 (Flow)

10 µg/mL

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产品规格

种属反应

Human, Mouse

已发表种属

Mouse, Rat

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to the C terminus amino acids NPTVLLKERSTEL (aa 592-604).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

TBS, pH 7.3, with 0.5% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2084941

产品详细信息

This antibody is predicted to react with bovine, canine and porcine based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.

靶标信息

COX2 converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis, including production of inflammatory prostaglandins. The conversion of arachidonate to prostaglandin H2 is a 2 step reaction: a cyclooxygenase (COX) reaction which converts arachidonate to prostaglandin G2 (PGG2) and a peroxidase reaction in which PGG2 is reduced to prostaglandin H2 (PGH2). It is constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and is up-regulated under pathological conditions, such as in cancer and inflammation (in contrast to the iso-enzyme PTGS1, which is expressed ubiquitously). Up-regulation of COX2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, COX2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. COX2 is naturally inhibited by calcitriol (the active form of Vitamin D). Glucocorticoids chronically trans-repress PTGS2 gene activity in vivo in part by interfering with transcription initiation and elongation. COX2 is a target of NSAID such as aspirin, which can reduce pain and swelling from inflammation driven by COX2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Novel aminopyridazine derivative of minaprine modified by radiolysis presents potent anti-inflammatory effects in LPS-stimulated RAW 264.7 and DH82 macrophage cells.

Scientific reports

Jeong GH,Lee H,Woo SY,Lee HK,Chung BY,Bai HW

PA1-9032 was used in Western Blotting to show a highly enhanced anti-inflammatory capacity inhibited nitric oxide (NO) and prostaglandin E2 (PGE2) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 and DH82 macrophage cells.

Wed Jul 05 00:00:00 UTC 2023

Prevention of Testicular Damage by Indole Derivative MMINA via Upregulated StAR and CatSper Channels with Coincident Suppression of Oxidative Stress and Inflammation: In Silico and In Vivo Validation.

Antioxidants (Basel, Switzerland)

Afsar T,Razak S,Trembley JH,Khan K,Shabbir M,Almajwal A,Alruwaili NW,Ijaz MU

Published figure using COX2 polyclonal antibody (Product # PA1-9032) in Western Blot

Wed Oct 19 00:00:00 UTC 2022

Sulindac acetohydrazide derivative attenuates against cisplatin induced organ damage by modulation of antioxidant and inflammatory signaling pathways.

Scientific reports

Razak S,Afsar T,Bibi N,Abulmeaty M,Bhat MA,Inam A,Trembley JH,Almajwal A,Shabbir M,Alruwaili NW,Algarni A

PA1-9032 was used in Western Blotting to illustrate that DMFM may affect protective action against cisplatin-induced brain, heart, liver, and kidney damage via reduction of inflammation and ROS.

Mon Jul 11 00:00:00 UTC 2022

Sulindac acetohydrazide derivative attenuates against cisplatin induced organ damage by modulation of antioxidant and inflammatory signaling pathways.

Scientific reports

Razak S,Afsar T,Bibi N,Abulmeaty M,Bhat MA,Inam A,Trembley JH,Almajwal A,Shabbir M,Alruwaili NW,Algarni A

PA1-9032 was used in Western Blotting to illustrate that DMFM may affect protective action against cisplatin-induced brain, heart, liver, and kidney damage via reduction of inflammation and ROS.

Mon Jul 11 00:00:00 UTC 2022

Korean Red Ginseng affects ovalbumin-induced asthma by modulating IL-12, IL-4, and IL-6 levels and the NF-κB/COX-2 and PGE₂ pathways.

Journal of ginseng research

Lee SY,Kim MH,Kim SH,Ahn T,Kim SW,Kwak YS,Cho IH,Nah SY,Cho SS,Park KM,Park DH,Bae CS

Published figure using COX2 polyclonal antibody (Product # PA1-9032) in Immunohistochemistry

Thu Jul 01 00:00:00 UTC 2021

Molecular docking, pharmacokinetic studies, and in vivo pharmacological study of indole derivative 2-(5-methoxy-2-methyl-1H-indole-3-yl)-N'-[(E)-(3-nitrophenyl) methylidene] acetohydrazide as a promising chemoprotective agent against cisplatin induced organ damage.

Scientific reports

Razak S,Afsar T,Bibi N,Abulmeaty M,Qamar W,Almajwal A,Inam A,Al Disi D,Shabbir M,Bhat MA

PA1-9032 was used in Flow Cytometry, Western Blot to investigate the mechanisms of action of indole derivative, 2-(5-methoxy-2-methyl-1H-indol-3-yl)-N'-[(E)-(3-nitrophenyl) methylidene] acetohydrazide (MMINA) against anticancer drug (cisplatin) induced organ damage using a rodent model.

Thu Mar 18 00:00:00 UTC 2021

Scientific reports

Razak S,Afsar T,Bibi N,Abulmeaty M,Qamar W,Almajwal A,Inam A,Al Disi D,Shabbir M,Bhat MA

Thu Mar 18 00:00:00 UTC 2021

Scientific reports

Razak S,Afsar T,Bibi N,Abulmeaty M,Qamar W,Almajwal A,Inam A,Al Disi D,Shabbir M,Bhat MA

Thu Mar 18 00:00:00 UTC 2021

Saururus chinensis-controlled allergic pulmonary disease through NF-κB/COX-2 and PGE₂ pathways.

PeerJ

Song M,Lee SY,Kim M,Park S,Park J,Kwon Y,Park DH

PA1-9032 was used in Immunohistochemistry-immunofluorescence to report findings on the use of Saururus chinensis as a promising drug candidate against fine dust-induced allergic pulmonary disease.

Fri Oct 09 00:00:00 UTC 2020

Saururus chinensis-controlled allergic pulmonary disease through NF-κB/COX-2 and PGE₂ pathways.

PeerJ

Song M,Lee SY,Kim M,Park S,Park J,Kwon Y,Park DH

PA1-9032 was used in Immunohistochemistry-immunofluorescence to report findings on the use of Saururus chinensis as a promising drug candidate against fine dust-induced allergic pulmonary disease.

Fri Oct 09 00:00:00 UTC 2020

Anti-inflammatory Effect of Curcuma longa and Allium hookeri Co-treatment via NF-κB and COX-2 Pathways.

Scientific reports

Lee SY,Cho SS,Li Y,Bae CS,Park KM,Park DH

Published figure using COX2 polyclonal antibody (Product # PA1-9032) in Immunofluorescence

Tue Mar 31 00:00:00 UTC 2020

Anti-inflammatory Effect of Curcuma longa and Allium hookeri Co-treatment via NF-κB and COX-2 Pathways.

Scientific reports

Lee SY,Cho SS,Li Y,Bae CS,Park KM,Park DH

Published figure using COX2 polyclonal antibody (Product # PA1-9032) in Immunofluorescence

Tue Mar 31 00:00:00 UTC 2020

生物信息学

蛋白别名: COX-2; cyclooxygenase; cyclooxygenase 2; cyclooxygenase 2b; Cyclooxygenase-2; Glucocorticoid-regulated inflammatory cyclooxygenase; Gripghs; Macrophage activation-associated marker protein P71/73; PES-2; PGH synthase 2; PHS II; Prostaglandin G/H synthase 2; Prostaglandin H2 synthase 2; Prostaglandin-endoperoxide synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase); TIS10 protein

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基因别名: COX-2; COX2; GRIPGHS; hCox-2; PGG/HS; PGHS-2; Pghs-b; Pghs2; PHS-2; PTGS2; TIS10

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UniProt ID: (Human) P35354, (Mouse) Q05769

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Entrez Gene ID: (Human) 5743, (Mouse) 19225

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功能