Invitrogen

COX3 Monoclonal Antibody (DA5BC4)

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COX3 Antibody (459300) in WB

Western blot analysis of COX3 in isolated heart mitochondria extracts using a COX3 Monoclonal Antibody (Product # 459300) at a concentration of 4 µg/mL. Lane 1 : Isolated mitochondria from Human heart at 5 µg, Lane 2 : Isolated mitochondria Rat heart at 10 µg, Lane 3 : Isolated mitochondria from Mouse heart at 10 µg. Predicted band size: 30 kDa.

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产品信息

459300

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2-4 µg/mL

查看4篇已发表文章 4篇已发表文章

产品规格

种属反应

Human, Mouse, Rat, Yeast

已发表种属

Yeast

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

DA5BC4

抗原

Full length native Yeast Cytochrome C Oxidase subunit III

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

IgG fraction

保存液

HEPES buffered saline

内含物

0.02% sodium azide

保存条件

4° C, do not freeze

运输条件

Wet ice

AB_10374345

产品详细信息

Positive control: Isolated mitochondria from Human , Rat and Mouse hearts.

WB Predicted molecular weight: 30 kDa.

靶标信息

COX3 is a multi-pass membrane protein. It belongs to the cytochrome c oxidase subunit 3 family. Defects in COX3 are a cause of Leber hereditary optic neuropathy (LHON) and cytochrome c oxidase deficiency (COX deficiency). Defects in MT-CO3 are also found in mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes (MELAS) syndrome and recurrent myoglobinuria.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Distinct Roles of Mitochondrial HIGD1A and HIGD2A in Respiratory Complex and Supercomplex Biogenesis.

Cell reports

Timón-Gómez A,Garlich J,Stuart RA,Ugalde C,Barrientos A

459300 was used in Western Blotting to illustrate the existence of multiple pathways to assemble these structures by dynamic HIGD-mediated CIV biogenesis.

Tue May 05 00:00:00 UTC 2020

The yeast mitochondrial proteins Rcf1 and Rcf2 support the enzymology of the cytochrome c oxidase complex and generation of the proton motive force.

The Journal of biological chemistry

Strogolova V,Hoang NH,Hosler J,Stuart RA

459300 was used in Western Blot to conclude that the slow aerobic growth of the Rcf1/Rcf2 double null mutant results from diminished generation of mitochondrial PMF by CIV and limits the level of CIV activity required for maintenance of the PMF and growth under aerobic conditions.