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CPLX1/2 Polyclonal Antibody

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CPLX1/2 Antibody (ANR-011-200UL) in WB

Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate: - 1. Anti-CPLX1/2 Antibody (#ANR-011), (1:200). 2. Anti-CPLX1/2 Antibody , preincubated with CPLX1/2 Blocking Peptide (#BLP-NR011).

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产品信息

ANR-011-200UL

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:200

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

(C)KDMGKMLGGDEEKD, corresponding to amino acid residues 14-27 of rat Complexin-1, Intracellular, N-terminus

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.8 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 1% BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

靶标信息

Complexins (1 through 4) are small cytosolic proteins that bind tightly to a complex of SNARE proteins to regulate synaptic vesicle membrane fusion via its interaction withsynaptotagmin. Complexins activate the quick release of neurotransmitters, triggered by calcium release. They also regulate the spontaneous release of neurotransmitters. Complexins are evolutionarily conserved in mammals, Drosophila, and Caenorhabditis elegans. They are divided into four structural domains: flexible N- and C-terminal domains, an accessory domain, and a central alpha-helical domain. The N-terminal domain plays a role in activation of fast synchronous release in murine neurons and in isolated chromaffin cells. The C-terminal domain binds to phospholipids and is important for regulating spontaneous release in neuronal cultures and suppressing Ca2+-independent fusion in vitro. The accessory domain is necessary for regulating spontaneous release and the central domain is required for all functions.

仅用于科研。不用于诊断过程。未经明确授权不得转售。