Invitrogen

CPT2 Polyclonal Antibody

CPT2 Antibody in Immunocytochemistry (ICC/IF)

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CPT2 Antibody (PA5-12217) in ICC/IF

Immunocytochemistry analysis of CPT2 in HepG2 cells. Samples were incubated in CPT2 polyclonal antibody (Product # PA5-12217) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Product Image Gallery

CPT2 Antibody — Generation of mice with a macrophage-specific loss of mitochondrial fatty acid ß-oxidation. A: mRNA for carnitine palmitoyltransferase (CPT) II (Cpt2) in bone marrow-derived macrophages (BMDM) from control [wild-type (WT)] mice and BMDM from mice with a specific knockout of Cpt2 [CPT2 Mɸ-KO (KO), n = 3]. B: Western blot for Cpt2 in control and CPT2 Mɸ-KO BMDM. C: Western blot of mitochondrial proteins in control and CPT2 Mɸ-KO BMDM in the presence and absence of IL-4 stimulation (n = 2). D: oxidation of [1-14C]oleic acid to 14CO2 in control and CPT2 Mɸ-KO BMDM (n = 6). E: oxidation of [U-14C]glucose to 14CO2 in control and CPT2 Mɸ-KO BMDM (n = 6). F: incorporation of [3H]acetate into lipid in control and CPT2 Mɸ-KO BMDM (n = 6). G: [1,2-3H]2-deoxyglucose uptake in control and CPT2 Mɸ-KO BMDM (n = 6). H: steady-state metabolite concentrations in IL-4- or LPS-IFNγ-stimulated control and CPT2 Mɸ-KO BMDM (n = 5-6). Values are means SE. *P < 0.05, ***P < 0.001 for genotype comparison. #P < 0.05, ###P < 0.001 for treatment comparison. {KO}

产品信息

PA5-12217

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫细胞化学 (ICC/IF)

1:10-1:50

免疫电泳 (IE)

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产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 431-462 amino acids from the C-terminal region of human CPT2

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2 mg/mL

纯化类型

Ammonium sulfate precipitation, Size-exclusion - Dialysis

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2292215

靶标信息

Carnitine palmitoyltransferase II precursor (CPT2) is a nuclear protein which is transported to the mitochondrial inner membrane. CPT2 together with carnitine palmitoyltransferase I oxidizes long-chain fatty acids in the mitochondria. Defects in this gene are associated with mitochondrial long-chain fatty-acid (LCFA) oxidation disorders.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Requirement of hepatic pyruvate carboxylase during fasting, high fat, and ketogenic diet.

The Journal of biological chemistry

Selen ES,Rodriguez S,Cavagnini KS,Kim HB,Na CH,Wolfgang MJ

PA5-12217 was used in Western Blotting to show that Pcx is important for maintaining the proper balance of pyruvate metabolism between oxidative and anaplerotic pathways.

Thu Dec 01 00:00:00 UTC 2022

HDAC inhibitors elicit metabolic reprogramming by targeting super-enhancers in glioblastoma models.

The Journal of clinical investigation

Nguyen TTT,Zhang Y,Shang E,Shu C,Torrini C,Zhao J,Bianchetti E,Mela A,Humala N,Mahajan A,Harmanci AO,Lei Z,Maienschein-Cline M,Quinzii CM,Westhoff MA,Karpel-Massler G,Bruce JN,Canoll P,Siegelin MD

PA5-12217 was used in Immunoelectrophoresis to investigate if glioblastoma (GBM) cultures and patients'' tumors harbor super-enhancers in several genes related to the Warburg effect.

Wed Jul 01 00:00:00 UTC 2020

Loss of macrophage fatty acid oxidation does not potentiate systemic metabolic dysfunction.

American journal of physiology. Endocrinology and metabolism

Gonzalez-Hurtado E,Lee J,Choi J,Selen Alpergin ES,Collins SL,Horton MR,Wolfgang MJ

PA5-12217 was used in Western Blotting to investigate the contribution of macrophage fatty acid oxidation directly to metabolic dysfunction in high-fat diet-induced obesity, by using mice with a myeloid-specific knockout of carnitine palmitoyltransferase II (CPT2 Mϕ-KO).

Mon May 01 00:00:00 UTC 2017

生物信息学

蛋白别名: Carnitine O-palmitoyltransferase 2, mitochondrial; Carnitine palmitoyltransferase II; CPT II; OTTHUMP00000010493; testicular secretory protein Li 13

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基因别名: AI323697; Cpt-2; CPT1; CPT2; CPTASE; CPTII; IIAE4

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UniProt ID: (Human) P23786, (Mouse) P52825

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Entrez Gene ID: (Human) 1376, (Mouse) 12896

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功能

carnitine O-palmitoyltransferase activity transferase activity transferase activity, transferring acyl groups acyltransferase

参与通路

fatty acid beta-oxidation carnitine shuttle lipid metabolic process fatty acid metabolic process transport