Invitrogen

CRBN Recombinant Rabbit Monoclonal Antibody (PSH0-69)

CRBN Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CRBN Antibody (MA5-49359) in IHC (P)

Immunohistochemical analysis of CRBN on formalin-fixed paraffin-embedded human testis tissue. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissue was blocked in 1% BSA for 20 minutes at room temperature, then probed with CRBN Rabbit Recombinant Monoclonal Antibody (PSH0-69) (Product # MA5-49359) at 1:200 dilution for 1 hour at room ... View More

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CRBN Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with CRBN siRNA and decrease in signal intensity was observed in Western Blot application using CRBN Rabbit Recombinant Monoclonal Antibody (PSH0-69) (Product # MA5-49359). {KD}

产品信息

MA5-49359

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化（石蜡） (IHC (P))

1:200

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

PSH0-69

抗原

Recombinant protein within human crbn aa 1-442 / 442.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_3075016

产品详细信息

Sequence Similarities: 95% Mouse/Rat.

Tissue Specificity: Widely expressed. Highly expressed in brain.

Positive Control: HEK-293 cell lysate, A375 cell lysate, A549 cell lysate, HeLa cell lysate, SH-SY5Y cell lysate, NIH/3T3 cell lysate, MCF7 cell lysate, mouse brain tissue lysate, rat testis tissue lysate, human testis tissue, human brain tissue.

Subcellular Location: Cytoplasm, Nucleus, Membrane.

Predicted band size: 51 kDa.

靶标信息

CRBN, a member of the Lon protease protein family, plays a role in brain development. It is a component of the DCX (DDB1-CUL4-X-box) E3 protein ligase complex, a complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins and is required for limb outgrowth and expression of the fibroblast growth factor FGF8. CRBN is thought to regulate the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. It is widely expressed and highly expressed in brain.

仅用于科研。不用于诊断过程。未经明确授权不得转售。