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CRM1 Antibody (703238) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of MCF7 (Lane 1), HeLa (Lane 2), SW480 (Lane 3), U-2 OS (Lane 4), PC-3 (Lane 5), A375 (Lane 6) and A549 (Lane 7). The blot was probed with Anti-CRM1 Recombinant Rabbit Monoclonal Antibody (Product # 703238, 1:2500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution). A ~123 kDa band corresponding to CRM1 was observed in all the cell lines tested.
产品信息
703238
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
8H4L7
抗原
Protein corresponding to human Exportin 1 [aa824-aa1071]
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2784570
产品详细信息
This antibody is predicted to react with Monkey, Bovine, Pig.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
Exportin - 1 (XPO1) mediates the nuclear export of cellular proteins bearing a leucine-rich nuclear export signal (NES) and RNAs. In the nucleus, in association with RANBP3, XPO1 binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex is mediated through binding to nucleoproteins. Upon transit of a nuclear complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP cause release of the cargo from the export receptor. The directionality of the nuclear export is thought to be conferred by an asymmetric distribution of the GTP and GDP bound forms of Ran between the cytoplasm and nucleus.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: chromosome region maintenance 1 homolog; Chromosome region maintenance 1 protein homolog; Exp1; exportin 1 (CRM1 homolog, yeast); Exportin-1; exportin-1 (required for chromosome region maintenance)
基因别名: CRM1; emb; exp1; XPO1
UniProt ID: (Human) O14980
Entrez Gene ID: (Human) 7514
ribosomal large subunit export from nucleus
ribosomal small subunit export from nucleus
negative regulation of transcription from RNA polymerase II promoter
protein export from nucleus
sister chromatid cohesion
regulation of centrosome duplication
viral process
protein localization to nucleus
regulation of protein catabolic process
response to drug
regulation of mRNA stability
regulation of protein export from nucleus
mRNA transport
intracellular transport of virus
