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CRYBA1 Antibody (PA5-97627) in ICC/IF
Immunofluorescent analysis of CRYBA1 in A549 cells using a CRYBA1 polyclonal antibody (Product # PA5-97627) at a dilution of 1:100. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.
产品信息
PA5-97627
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant Human Beta-crystallin A3 protein (1-215AA)
偶联物
形式
Liquid
浓度
3 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.03% ProClin 300
保存条件
-20°C or -80°C if preferred
运输条件
Wet ice
RRID
AB_2812242
靶标信息
Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta acidic group member, encodes two proteins (crystallin, beta A3 and crystallin, beta A1) from a single mRNA, the latter protein is 17 aa shorter than crystallin, beta A3 and is generated by use of an alternate translation initiation site. Deletion of exons 3 and 4 causes the autosomal dominant disease 'zonular cataract with sutural opacities'.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: beta crystallin A3 chain transcript CN; beta crystallin A3 chain transcript LAM; beta crystallin A3 chain transcript PS; beta crystallin A3 chain transcript TC; beta-A1-crystallin; beta-A3/A1-crystallin; beta-crystallin A1; Beta-crystallin A3; crystallin beta A3; crystallin beta A3/A1; crystallin, beta A1; crystallin, beta A3; eye lens structural protein; truncated beta-crystallin A3
基因别名: BA3/A1; Cryb; CRYB1; CRYBA1; CTRCT10
UniProt ID: (Human) P05813
Entrez Gene ID: (Human) 1411, (Mouse) 12957
visual perception
camera-type eye development
negative regulation of cytokine production
lens development in camera-type eye
negative regulation of phosphatidylinositol 3-kinase signaling
negative regulation of TOR signaling
negative regulation of protein kinase B signaling
negative regulation of ERK1 and ERK2 cascade
positive regulation of anoikis
