Invitrogen

CSF1R Polyclonal Antibody

CSF1R Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 7

CSF1R Antibody (PA5-25974) in ICC/IF

Immunofluorescence analysis of CSF1R was performed using 70% confluent log phase THP-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with anti-CSF1R Polyclonal Antibody (Product # PA5-25974) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product #... View More

CSF1R Antibody in Immunohistochemistry, Immunohistochemistry (Frozen) (IHC, IHC (F))

CSF1R Antibody in Immunohistochemistry (IHC)

Product Image Gallery

产品信息

PA5-25974

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:10-1:50

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

1:10-1:50

产品规格

种属反应

Human, Mouse

已发表种属

Horse, Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

CSF1R recombinant protein.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2543474

靶标信息

CSF1R (FMS) is involved in the production, differentiation, and function of macrophages, and is implicated in promyelocytic leukemias. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. CSF1R is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in the gene encoding CSF1R have been associated with a predisposition to myeloid malignancy.Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and plays an important role in innate immunity and in inflammatory processes. The first intron of the CSF1R gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Mutations in the CSF1R gene have been associated with a predisposition to myeloid malignancy.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Characterisation of the testicular transcriptome in stallions with age-related testicular degeneration.

Equine veterinary journal

Woodward E,Schlingmann K,Tobias J,Turner R

PA5-25974 was used in Flow Cytometry, Immunocytochemistry, Western Blot, Immunohistochemistry to suggest that chronic, low-grade inflammation may be involved in the pathophysiology of age-related testicular degeneration in stallions.

Wed Mar 01 00:00:00 UTC 2023

Characterisation of the testicular transcriptome in stallions with age-related testicular degeneration.

Equine veterinary journal

Woodward E,Schlingmann K,Tobias J,Turner R

Wed Mar 01 00:00:00 UTC 2023

Minocycline suppresses disease-associated microglia (DAM) in a model of photoreceptor cell degeneration.

Experimental eye research

Ozaki E,Delaney C,Campbell M,Doyle SL

PA5-25974 was used in Western Blot to show that minocycline suppresses many DAM markers, in particular those associated with lipid metabolism indicating that suppression of this process is one mechanism by which minocycline protects against inflammation induced photoreceptor cell death.

Fri Apr 01 00:00:00 UTC 2022

Evidence of a Myenteric Plexus Barrier and Its Macrophage-Dependent Degradation During Murine Colitis: Implications in Enteric Neuroinflammation.

Cellular and molecular gastroenterology and hepatology

Dora D,Ferenczi S,Stavely R,Toth VE,Varga ZV,Kovacs T,Bodi I,Hotta R,Kovacs KJ,Goldstein AM,Nagy N

PA5-25974 was used in Flow Cytometry, Immunocytochemistry, Western Blot, Immunohistochemistry to suggest a potential mechanism for the onset of neuroinflammation in colitis and other GI pathologies with acquired enteric neuronal dysfunction.

Mon Mar 28 00:00:00 UTC 2022

Evidence of a Myenteric Plexus Barrier and Its Macrophage-Dependent Degradation During Murine Colitis: Implications in Enteric Neuroinflammation.

Cellular and molecular gastroenterology and hepatology

Dora D,Ferenczi S,Stavely R,Toth VE,Varga ZV,Kovacs T,Bodi I,Hotta R,Kovacs KJ,Goldstein AM,Nagy N

Mon Mar 28 00:00:00 UTC 2022

Evidence of a Myenteric Plexus Barrier and Its Macrophage-Dependent Degradation During Murine Colitis: Implications in Enteric Neuroinflammation.

Cellular and molecular gastroenterology and hepatology

Dora D,Ferenczi S,Stavely R,Toth VE,Varga ZV,Kovacs T,Bodi I,Hotta R,Kovacs KJ,Goldstein AM,Nagy N

Mon Mar 28 00:00:00 UTC 2022

Attenuated CSF-1R signalling drives cerebrovascular pathology.

EMBO molecular medicine

Delaney C,Farrell M,Doherty CP,Brennan K,O'Keeffe E,Greene C,Byrne K,Kelly E,Birmingham N,Hickey P,Cronin S,Savvides SN,Doyle SL,Campbell M

PA5-25974 was used in Immunocytochemistry-immunofluorescence to suggest a targeted approach to restoring BBB integrity while also restoring CSF-1R signalling in macrophages could drive a therapeutic response in ALSP patients and other AD-like dementia.

Fri Feb 05 00:00:00 UTC 2021

生物信息学

蛋白别名: c-fms; CD115; CD115 antigen; CSF-1 receptor; CSF-1-R; CSFIR; EC 2.7.10.1; FMS proto-oncogene; kinase CSFR; macrophage colony stimulating factor I receptor; Macrophage colony-stimulating factor 1 receptor; McDonough feline sarcoma viral (v-fms) oncogene homolog; OTTHUMP00000224093; Proto-oncogene c-Fms; proto-oncogene fms

查看更多收起

基因别名: AI323359; C-FMS; CD115; CSF-1R; CSF1R; Csfmr; CSFR; Fim-2; FIM2; FMS; HDLS; M-CSF-R; M-CSFR

查看更多收起

UniProt ID: (Human) P07333, (Mouse) P09581

查看更多收起

Entrez Gene ID: (Human) 1436, (Mouse) 12978

查看更多收起

功能