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CSF2RB Chimeric Antibody (MA5-48120) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of CD131 (CSF2RB) in U937 cells using CSF2RB Chimeric Monoclonal Antibody (Product # MA5-48120). Paraformaldehyde-fixed cells were immobilized on cover slips and stained with CSF2RB Chimeric Monoclonal Antibody at 10 µg/mL followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from lef... View More
产品信息
MA5-48120
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
BION-1
抗原
BALB/c mice were immunized intraperitonally with COS cells transfected DQP (Domain 4 of the common beta-chain) expression constructs.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.02% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_3074563
产品详细信息
Specificity: Binds to domain 4 of the common beta-chain and recognises the same binding site used by IL-5, GM-CSF and IL-3, or binds to an epitope in close proximity to it. Residues M363, R364, E366 (B-C loop) and R418 (F-G loop) in the common beta-chain are important for BION-1 binding.
Preparation: BALB/c mice were immunized intraperitonally with COS cells transfected DQP (Domain 4 of the common beta-chain) expression constructs. The immunizations were repeated 4 times at 2-week intervals. Four weeks after the final immunization, a mouse was boosted with COS transfectants intravenously. Three days later, splenocytes were harvested and fused with NS-1 myeloma cells. Hybridoma supernatants were screened on CHO DQP cells by flow cytometry, with untransfected CHO cells as a control. All antibodies were from single hybridoma clones as selected by a limiting dilution method. MoAbs were purified from ascites fluid or hybridoma supernatant by a protein A sepharose column.
靶标信息
The protein encoded by this gene is the common beta chain of the high affinity receptor for IL-3, IL-5 and CSF. Defects in this gene have been reported to be associated with protein alveolar proteinosis (PAP).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: beta common cytokine receptor; CD antigen CD131; CD131; CDw131; colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage); colony-stimulating factor-2 receptor, beta, low-affinity; Cytokine receptor common subunit beta; GM-CSF/IL-3/IL-5 receptor common beta subunit; GM-CSF/IL-3/IL-5 receptor common beta-chain; IL-3 RB; IL-3 Receptor beta; IL3R beta; interleukin 3 receptor/granulocyte-macrophage colony stimulating factor 3 receptor, beta (high affinity)
基因别名: CD131; CDw131; CSF2RB; IL3RB; IL5RB; SMDP5
UniProt ID: (Human) P32927
Entrez Gene ID: (Human) 1439
