Invitrogen

CTLA-4 (CD152) Chimeric Recombinant Rabbit Monoclonal Antibody (9H10)

CTLA-4 (CD152) Chimeric Antibody in ELISA (ELISA)

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CTLA-4 (CD152) Chimeric Antibody (MA5-48106) in ELISA

ELISA analysis of CTLA-4 (CD152) chimeric monoclonal antibody (Product # MA5-48106) (red line) and isotype control (blue line) was performed by coating plate with CTLA-4 (CD152)-Fc fusion protein at a concentration of 5 µg/mL. A 3-fold serial dilution from 10,000 to 0.17 ng/mL of the antibody was done. For signal detection, a 1:4,000 dilution of HRP-labelled anti-rabbit IgG1 antibody was used.

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产品信息

MA5-48106

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

流式细胞分析 (Flow)

Assay-dependent

酶联免疫吸附实验 (ELISA)

0.17 ng/mL

中和实验 (Neu)

Assay-dependent

产品规格

种属反应

Mouse

宿主/亚型

Rabbit / IgG, kappa

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

9H10

抗原

This antibody was raised by immunising Syrian hamsters with Staphylococcus A bacteria coated in CTLA-4, isolating B cells from the immunised hamsters and fusing these with the P3X3.Ag8.653 myeloma line to produce stable hybridomas.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.02% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_3074549

产品详细信息

Specificity: This antibody is specific for murine CTLA-4, an inhibitory receptor that acts as the primary negative regulator of T-cell responses. CTLA-4 is expressed predominantly by activated T cells, with significantly higher levels of expression on CD8+ T cells than CD4+ T cells.

靶标信息

CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor expressed on mainly CD4+ T cells. It binds the same ligands as CD28 (CD80 and CD86 on B cells and dendritic cells), but with higher affinity than CD28. However, in contrast to CD28 which enhances cell function when bound at the same time as the T cell receptor, CTLA4 inhbits the T cell and prevents it from functioning. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function.

仅用于科研。不用于诊断过程。未经明确授权不得转售。