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CTU2 Antibody (PA5-88908) in WB
Western blot analysis of CTU2 in extracts of various cell lines. Samples were incubated with CTU2 Polyclonal antibody (Product # PA5-88908) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
产品信息
PA5-88908
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 186-515 of human CTU2 (NP_0010127771)
偶联物
形式
Liquid
浓度
1.72 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2805212
产品详细信息
Immunogen sequence: HVLGAGGGPG PTQGEEQPPQ PPLDPQNLAR PPAPAQTEAL SQLFCSVRTL TAKEELLQTL RTHLILHMAR AHGYSKVMTG DSCTRLAIKL MTNLALGRGA FLAWDTGFSD ERHGDVVVVR PMRDHTLKEV AFYNRLFSVP SVFTPAVDTK APEKASIHRL MEAFILRLQT QFPSTVSTVY RTSEKLVKGP RDGPAAGDSG PRCLLCMCAL DVDAADSATA FGAQTSSRLS QMQSPIPLTE TRTPPGPCCS PGVGWAQRCG QGACRREDPQ ACIEEQLCYS CRVNMKDLPS LDPLPPYILA EAQLRTQRAW GLQEIRDCLI EDSDDEAGQS; Positive Samples: A-431, Jurkat; Cellular Location: Cytoplasm
靶标信息
Cytoplasmic tRNA 2-thiolation protein 2 (CUT2), also named as C16orf84 or NCS2, is a 515 amino acid protein, which belongs to the CUT2 family. C16orf84 localizes in the cytoplasm and Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). C16orf84 may act by forming a heterodimer with CTU1/ATPBD3 that ligates sulfur from thio-carboxylated URM1 onto the uridine of tRNAs at wobble position.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Cytoplasmic tRNA 2-thiolation protein 2; Cytosolic thiouridylase subunit 2; cytosolic thiouridylase subunit 2 homolog
基因别名: C16orf84; CTU2; NCS2; UPF0432
UniProt ID: (Human) Q2VPK5
Entrez Gene ID: (Human) 348180
