Invitrogen

CX3CR1 Recombinant Rabbit Monoclonal Antibody (1H14L7)

CX3CR1 Antibody in Immunocytochemistry (ICC/IF)

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CX3CR1 Antibody (702321) in ICC/IF

For immunofluorescence analysis, THP-1 cells were fixed and permeabilized for detection of endogenous CX3CR1 using Anti-CX3CR1 Recombinant Rabbit Monoclonal Antibody (Product # 702321, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of CX3CR1 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S3... View More

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产品信息

702321

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

2 µg/mL

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

1H14L7

抗原

Peptides corresponding to human CX3CR1 (aa6-19, 339-351)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2662897

产品详细信息

This antibody is predicted to react with Monkey, Dog, Cat

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

CX3CR1 is one of the chemokine receptors that are required as coreceptors for HIV infection. The genes encoding human, mouse, and rat CX3CR1 were cloned and designated V28 and CMKBRL1, CX3CR1, and RBS11, respectively. The encoded seven transmembrane protein was recently identified as the receptor for a novel transmembrane molecule, fractalkine, and renamed CX3CR1. Recently, CX3CR1 was found to serve as a coreceptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by fractokine. CX3CR1 mediates leukocyte migration an d adhesion. CX3CR1 is expressed in a variety of human tissues and cell lines.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Continuous administration of mirabegron has advantages in inhibition of central sensitization compared with short-term treatment cessation in a mouse model of overactive bladder.

Neurourology and urodynamics

Kwon J,Lee EJ,Park HR,Cho HJ,Jang JA,Yang H,An J,Park D,Kim YJ,Hur KJ,Kim JS,Yoshimura N

702321 was used in Immunocytochemistry, Western Blot, Immunohistochemistry to suggest that continuous mirabegron treatment seems to prevent central sensitization and, thus, might be desirable for long-term disease control of overactive bladder (OAB).

Mon Aug 01 00:00:00 UTC 2022

Circadian Regulator CLOCK Recruits Immune-Suppressive Microglia into the GBM Tumor Microenvironment.

Cancer discovery

Chen P,Hsu WH,Chang A,Tan Z,Lan Z,Zhou A,Spring DJ,Lang FF,Wang YA,DePinho RA

702321 was used in Immunohistochemistry, Immunocytochemistry to elucidate a novel function for CLOCK in supporting an immune suppressive TME via its upregulation of OLFML3.

Sun Mar 01 00:00:00 UTC 2020

Circadian Regulator CLOCK Recruits Immune-Suppressive Microglia into the GBM Tumor Microenvironment.

Cancer discovery

Chen P,Hsu WH,Chang A,Tan Z,Lan Z,Zhou A,Spring DJ,Lang FF,Wang YA,DePinho RA

702321 was used in Immunohistochemistry, Immunocytochemistry to elucidate a novel function for CLOCK in supporting an immune suppressive TME via its upregulation of OLFML3.

Sun Mar 01 00:00:00 UTC 2020

生物信息学

蛋白别名: Beta chemokine receptor-like 1; C-X3-C CKR-1; chemokine (C-C) receptor-like 1; chemokine (C-X3-C motif) receptor 1; chemokine (C-X3-C) receptor 1; CMK-BRL-1; CMK-BRL1; Cx3c; CX3C chemokine receptor 1; Fractalkine receptor; G protein-coupled receptor 13; G-protein coupled receptor 13; Scyd1; V28

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基因别名: CCRL1; CMKBRL1; CMKDR1; CX3CR1; GPR13; GPRV28; Rbs11; V28

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UniProt ID: (Human) P49238, (Rat) P35411, (Mouse) Q9Z0D9

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Entrez Gene ID: (Human) 1524, (Rat) 171056, (Mouse) 13051

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功能

chemokine receptor activity protein binding C-X3-C chemokine receptor activity C-X3-C chemokine binding cytokine receptor activity signal transducer activity G-protein coupled receptor activity

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