Invitrogen

CXCL1 Recombinant Superclonal™ Antibody (14HCLC)

CXCL1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 3

CXCL1 Antibody (712317) in ICC/IF

For immunofluorescence analysis, A375 cells were fixed and permeabilized for detection of endogenous CXCL1 using Anti-CXCL1 Recombinant Rabbit Superclonal™ Antibody (Product # 712317, 1:100) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962) and cytoskeletal F-actin (red) staining using Rhodamine Phalloidin (Product # R415, 1:300) Panel a-d) shows represent... View More

Product Image Gallery

CXCL1 Antibody — Detection of subcellular localization of the target protein by cell treatment demonstrates antibody specificity. Immunofluorescence analysis of CXCL1 using Anti-CXCL1 Recombinant Rabbit Superclonal™ Antibody (Product # 712317) detects CXCL1 in the cytoplasm of A375 cells treated with TNF alpha in comparison to control cells. {TM}

产品信息

712317

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

1:100

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Superclonal

类型

Antibody

克隆号

14HCLC

抗原

Peptide corresponding to human CXCL1 [aa99-aa107]

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2784615

产品详细信息

This Antibody is predicted to react with Monkey, Pig, Sheep, Bovine.

Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

靶标信息

CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Irisin Stimulates the Release of CXCL1 From Differentiating Human Subcutaneous and Deep-Neck Derived Adipocytes via Upregulation of NFκB Pathway.

Frontiers in cell and developmental biology

Shaw A,Tóth BB,Király R,Arianti R,Csomós I,Póliska S,Vámos A,Korponay-Szabó IR,Bacso Z,Győry F,Fésüs L,Kristóf E

712317 was used in Immunocytochemistry to demonstrate that irisin stimulates the release of a novel adipokine, CXCL1, via upregulation of NFκB pathway in neck area derived adipocytes, which might play an important role in improving tissue vascularization.

Sat Oct 30 00:00:00 UTC 2021

Irisin Stimulates the Release of CXCL1 From Differentiating Human Subcutaneous and Deep-Neck Derived Adipocytes via Upregulation of NFκB Pathway.

Frontiers in cell and developmental biology

Shaw A,Tóth BB,Király R,Arianti R,Csomós I,Póliska S,Vámos A,Korponay-Szabó IR,Bacso Z,Győry F,Fésüs L,Kristóf E

Sat Oct 30 00:00:00 UTC 2021

生物信息学

蛋白别名: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 1; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha); CXC; CXC motif chemokine; CXCL; CXCL-1; fibroblast secretory protein; GRO a; GRO α; GRO-alpha(1-73); GRO1 oncogene (melanoma growth stimulating activity, alpha); GRO1 oncogene (melanoma growth-stimulating activity); GROalpha; Growth-regulated alpha protein; GROα; melanoma growth stimulating activity, alpha; Melanoma growth stimulatory activity; melanoma growth stimulatory activity alpha; MGSA; MGSA alpha; NAP-3; NAP3; Neutrophil-activating protein 3

查看更多收起

基因别名: CXCL1; FSP; GRO; GRO1; GROA; MGSA; MGSA-a; NAP-3; SCYB1

查看更多收起

UniProt ID: (Human) P09341

查看更多收起

Entrez Gene ID: (Human) 2919

查看更多收起

功能

receptor binding chemokine activity enzyme activator activity growth factor activity CXCR chemokine receptor binding chemokine

参与通路