Invitrogen

CXCL10 Recombinant Rabbit Monoclonal Antibody (10H11L3)

CXCL10 Antibody in Immunocytochemistry (ICC/IF)

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CXCL10 Antibody (701225) in ICC/IF

Immunofluorescence analysis of C-X-C motif chemokine 10 was performed using 70% confluent log phase HT-29 cells and HT-29 cells treated with 50 ng/mL IFN gamma for 14 hr followed by 1x PTI treatment for 4h. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CXCL10 Recombinant Rabbit Monoclonal Antibody (10H11L3) (Product # 701225) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnig... View More

CXCL10 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

701225

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:20

免疫细胞化学 (ICC/IF)

1:200

流式细胞分析 (Flow)

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产品规格

种属反应

Human, Mouse

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

10H11L3

抗原

proprietary

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2532429

产品详细信息

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

IP-10 (interferon-gamma induced protein 10 kD; in mouse, also called cytokine responsive gene 2, Crg-2, or mob-1) is a highly inducible, primary response gene that belongs to the CXC chemokine superfamily. It was first cloned in 1985. The biological functions of IP-10 are still unclear. Like Mig, IP-10 has no activity on neutrophils. Its functions include stimulation of monocytes, natural killer and T-cell migration, regulation of T-cell and bone marrow progenitor maturation, modulation of adhesion molecule expression as well as inhibition of angiogenesis. Mig and IP-10 share the same receptor, CXCR3.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Sensory neuron dysfunction in orthotopic mouse models of colon cancer.

Journal of neuroinflammation

Balogh M,Zhang J,Gaffney CM,Kalakuntla N,Nguyen NT,Trinh RT,Aguilar C,Pham HV,Milutinovic B,Nichols JM,Mahalingam R,Shepherd AJ

701225 was used in Immunohistochemistry to demonstrate that colon cancer is associated with latent sensory neuron dysfunction and implicates cytokine/chemokine signaling in this process.

Fri Aug 12 00:00:00 UTC 2022

Loss of APP in mice increases thigmotaxis and is associated with elevated brain expression of IL-13 and IP-10/CXCL10.

Physiology & behavior

Mayagoitia K,Tolan AJ,Shammi S,Shin SD,Menchaca JA,Figueroa JD,Wilson CG,Bellinger DL,Ahmed ASI,Soriano S

701225 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to show that AppKO mice, compared to wildtype controls, experienced increased thigmotactic behavior but no memory impairments, and this phenotype correlated with increased IP-10 and IL-13 levels.

Fri Oct 15 00:00:00 UTC 2021

Immune synapses between mast cells and γδ T cells limit viral infection.

The Journal of clinical investigation

Mantri CK,St John AL

701225 was used in Flow cytometry/Cell sorting to show that immune synapse formation between MCs and γδ T cells is a novel mechanism to induce specific and protective immunity at sites of viral infection.

Fri Mar 01 00:00:00 UTC 2019

BPIFA1 regulates lung neutrophil recruitment and interferon signaling during acute inflammation.

American journal of physiology. Lung cellular and molecular physiology

Britto CJ,Niu N,Khanal S,Huleihel L,Herazo-Maya JD,Thompson A,Sauler M,Slade MD,Sharma L,Dela Cruz CS,Kaminski N,Cohn LE

701225 was used in Immunohistochemistry to identify a novel role for Bpifa1 in the regulation of Cxcl10-mediated PMN recruitment to the lungs via IFNγ and -λ signaling during acute inflammation.

Fri Feb 01 00:00:00 UTC 2019

生物信息学

蛋白别名: 10 kDa interferon gamma-induced protein; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 10; C7; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 10; CXC; CXC motif chemokine; CXCL; gamma IP10; Gamma-IP10; H-IP-10; interferon activated gene 10; Interferon-gamma induced protein CRG-2; interferon-gamma-induced protein CRG-2; interferon-inducible cytokine IP-10; M-IP-10; protein 10 from interferon (gamma)-induced cell line; small inducible cytokine B subfamily (Cys-X-Cys), member 10; small inducible cytokine subfamily B (Cys-X-Cys), member 10; Small-inducible cytokine B10

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基因别名: C7; CRG-2; Crg2; CXCL10; gIP-10; IFI10; INP10; IP-10; IP10; mob-1; SCYB10

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UniProt ID: (Human) P02778, (Mouse) P17515

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Entrez Gene ID: (Human) 3627, (Mouse) 15945

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功能

receptor binding protein binding chemokine activity heparin binding cAMP-dependent protein kinase regulator activity CXCR3 chemokine receptor binding cytokine activity chemokine

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