Invitrogen

CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody, PeproTech®

CXCL12 alpha (SDF-1 alpha) Antibody in Western Blot (WB)

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CXCL12 alpha (SDF-1 alpha) Antibody (500-P164G-1MG) in WB

Western Blot: To detect Murine SDF-1alpha (CXC12) by Western Blot analysis CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody (Product # 500-P164G-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine SDF-1alpha (CXCL12) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

CXCL12 alpha (SDF-1 alpha) Antibody in ELISA (ELISA)

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产品信息

500-P164G-1MG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

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酶联免疫吸附实验 (ELISA)

0.5-2.0 µg/mL

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体外检测 (IV)

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免疫染色 (IS)

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Goat

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived Recombinant Murine SDF-1alpha (CXCL12)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

AB_2929582

产品详细信息

AA Sequence of recombinant protein: KPVSLSYRCP CRFFESHIAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK.

Preparation: Produced from sera of goats immunized with highly pure recombinant Murine SDF-1alpha (CXCL12). Anti-Murine SDF-1alpha (CXCL12)-specific antibody was purified by affinity chromatography employing an immobilized Murine SDF-1alpha (CXCL12) matrix.

Sandwich ELISA: To detect Murine SDF-1alpha (CXCL12) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine SDF-1alpha (CXCL12) (500-P164GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine SDF-1alpha (CXCL12).

Western Blot: To detect Murine SDF-1alpha (CXC12) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine SDF-1alpha (CXCL12) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

靶标信息

CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Application of light sheet microscopy for qualitative and quantitative analysis of bronchus-associated lymphoid tissue in mice.

Cellular & molecular immunology

Mzinza DT,Fleige H,Laarmann K,Willenzon S,Ristenpart J,Spanier J,Sutter G,Kalinke U,Valentin-Weigand P,Förster R

Mon Oct 01 00:00:00 UTC 2018

IL-17-induced CXCL12 recruits B cells and induces follicle formation in BALT in the absence of differentiated FDCs.

The Journal of experimental medicine

Fleige H,Ravens S,Moschovakis GL,Bölter J,Willenzon S,Sutter G,Häussler S,Kalinke U,Prinz I,Förster R

Mon Apr 07 00:00:00 UTC 2014

IL-17-induced CXCL12 recruits B cells and induces follicle formation in BALT in the absence of differentiated FDCs.

The Journal of experimental medicine

Fleige H,Ravens S,Moschovakis GL,Bölter J,Willenzon S,Sutter G,Häussler S,Kalinke U,Prinz I,Förster R

Mon Apr 07 00:00:00 UTC 2014

FGF2 posttranscriptionally down-regulates expression of SDF1 in bone marrow stromal cells through FGFR1 IIIc.

Blood

Nakayama T,Mutsuga N,Tosato G

Thu Feb 15 00:00:00 UTC 2007

FGF2 posttranscriptionally down-regulates expression of SDF1 in bone marrow stromal cells through FGFR1 IIIc.

Blood

Nakayama T,Mutsuga N,Tosato G

Thu Feb 15 00:00:00 UTC 2007

Regulatory T cells interfere with the development of bronchus-associated lymphoid tissue.

The Journal of experimental medicine

Kocks JR,Davalos-Misslitz AC,Hintzen G,Ohl L,Förster R

Mon Apr 16 00:00:00 UTC 2007

Binding of lymphoid chemokines to collagen IV that accumulates in the basal lamina of high endothelial venules: its implications in lymphocyte trafficking.

Journal of immunology (Baltimore, Md. : 1950)

Yang BG,Tanaka T,Jang MH,Bai Z,Hayasaka H,Miyasaka M

Mon Oct 01 00:00:00 UTC 2007

CUTL1 is a target of TGF(beta) signaling that enhances cancer cell motility and invasiveness.

Cancer cell

Michl P,Ramjaun AR,Pardo OE,Warne PH,Wagner M,Poulsom R,D'Arrigo C,Ryder K,Menke A,Gress T,Downward J

Wed Jun 01 00:00:00 UTC 2005

生物信息学

蛋白别名: 12-O-tetradecanoylphorbol 13-acetate repressed protein 1; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 12; CC motif chemokine; CCmotif chemokine; CXC; CXC motif chemokine; CXCL; H-SDF-1-alpha; PBSF; Pre-B cell growth-stimulating factor; pre-B-cell growth-stimulating factor; SDF 1 a; SDF 1 alpha; SDF 1α; SDF-1; SDF1 alpha; SDF1α; Stromal cell-derived factor 1; stromal cell-derived factor-1 alpha; Thymic lymphoma cell-stimulating factor; TLSF; TPAR1

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基因别名: Cxcl12; Pbsf; Scyb12; Sdf1; Tlsf; Tpar1

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UniProt ID: (Mouse) P40224

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Entrez Gene ID: (Mouse) 20315

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功能

cytokine activity chemokine activity growth factor activity chemokine receptor binding CXCR chemokine receptor binding

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