Invitrogen

CXCL13 Monoclonal Antibody (DS8CX13), APC, eBioscience™

CXCL13 Antibody in Flow Cytometry (Flow)

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CXCL13 Antibody (17-7981-82) in Flow

BALB/c mouse resident peritoneal macrophages were stained with CD11b Monoclonal Antibody, FITC (Product # 11-0112-82) and F4/80 Monoclonal Antibody, eFluor 450 (Product # 48-4801-82). The cells were then fixed and permeabilized using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, and stained with 0.06 µg of Rat IgG2a kappa Isotype Control, APC (Product # 17-4321-81) (left) or 0.06 µg of CXCL13 Monoclonal Antibody, APC (right). CD11b positive cells were used for analysis.

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产品信息

17-7981-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

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流式细胞分析 (Flow)

0.125 µg/test

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

DS8CX13

抗原

Full length CXCL13

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偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2762702

产品详细信息

Description: This DS8CX13 monoclonal antibody recognizes mouse CXCL13 also known as BCA-1 and Angie.

Applications Reported: This DS8CX13 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This DS8CX13 antibody has been tested by flow cytometric analysis of resident peritoneal macrophages. This may be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser

靶标信息

CXCL13, also known as BCA-1 and Angie, is a small chemokine strongly expressed in the secondary lymphoid organs. It preferentially promotes the migration of B lymphocytes (both B-1 and B-2) to the follicles of the spleen, lymph nodes, and Peyer's patches. It is produced by macrophages, and in mouse the highest constitutive producers include large peritoneal macrophages, Kuppfer cells and lung macrophages (both CD11c+ and CD11c-). The cellular ligand for CXCL13 is CXCR5 also known as BLR-1 (Burkitt’s Lymphoma Receptor 1). CXCR5 is expressed on B cells, Tfh cells and, to a lower degree, some memory T cell populations. CXCL13 plays an important role in the development of chronic lymphocytic leukemia (CCL). CXCL13 is also a plasma biomarker for germinal center activity and generation of broadly neutralizing antibodies against HIV.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Role of CXCL5 in Regulating Chemotaxis of Innate and Adaptive Leukocytes in Infected Lungs Upon Pulmonary Influenza Infection.

Frontiers in immunology

Guo L,Li N,Yang Z,Li H,Zheng H,Yang J,Chen Y,Zhao X,Mei J,Shi H,Worthen GS,Liu L

17-7981-82 was used in Flow cytometry/Cell sorting to demonstrate CXCL5 affects B lymphocyte accumulation in the lungs by regulating the expression of the CXCL13.

Tue Feb 08 00:00:00 UTC 2022

Role of CXCL5 in Regulating Chemotaxis of Innate and Adaptive Leukocytes in Infected Lungs Upon Pulmonary Influenza Infection.

Frontiers in immunology

Guo L,Li N,Yang Z,Li H,Zheng H,Yang J,Chen Y,Zhao X,Mei J,Shi H,Worthen GS,Liu L

17-7981-82 was used in Flow cytometry/Cell sorting to demonstrate CXCL5 affects B lymphocyte accumulation in the lungs by regulating the expression of the CXCL13.

Tue Feb 08 00:00:00 UTC 2022

Stromal Cells Covering Omental Fat-Associated Lymphoid Clusters Trigger Formation of Neutrophil Aggregates to Capture Peritoneal Contaminants.

Immunity

Jackson-Jones LH,Smith P,Portman JR,Magalhaes MS,Mylonas KJ,Vermeren MM,Nixon M,Henderson BEP,Dobie R,Vermeren S,Denby L,Henderson NC,Mole DJ,Bénézech C

17-7981 was used in Immunohistochemistry-immunofluorescence to show how specialized omental mesothelial cells coordinate the recruitment and aggregation of neutrophils to capture peritoneal contaminants.

Tue Apr 14 00:00:00 UTC 2020

生物信息学

蛋白别名: B lymphocyte chemoattractant; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 13; CC motif chemokine; CCmotif chemokine; chem; CXC; CXC chemokine BLC; CXC motif chemokine; CXC motif chemokine 13; CXCL; small inducible cytokine subfamily B (Cys-X-Cys), member 13; Small-inducible cytokine B13

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基因别名: 4631412M08Rik; Angie; ANGIE2; BCA-1; BLC; BLR1L; Cxcl13; Scyb13

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UniProt ID: (Mouse) O55038

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Entrez Gene ID: (Mouse) 55985

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功能