Invitrogen

CXCL2 Recombinant Rabbit Monoclonal Antibody (16H3L10)

CXCL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CXCL2 Antibody (701126) in IHC (P)

Immunohistochemistry analysis of CXCL2/MIP-2 showing staining in the cytoplasm of paraffin-embedded mouse spleen tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperatu... View More

CXCL2 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CXCL2 Antibody — Figure 8 Fisetin reduces inflammation and expression of CXCLs in LPS-induced RAW 264.7 cells. RAW 264.7 cells were pre-treated with LPS (100 ng/ml) for 2 h, followed by treatment with fisetin (15 or 30 u M) for another 24 h. (A) Flow cytometric analysis was used to quantify CD3-expressing cells. (B) CD3 expression levels were measured using western blot analysis. (C) Reverse transcription-quantitative polymerase chain reaction analysis of pro-inflammatory cytokines as indicated. (D) CXCL-1, CXCL-2, CCL-3 and CXCR-2 expression levels were assessed using western blot analysis. Values are expressed as the mean +- standard error of the mean (n=8 in each group). *** P<0.001 vs. the Con group; + P<0.05, ++ P<0.01 and +++ P<0.001 vs. the LPS group. Con, control; LPS, lipopolysaccharide; CXC, chemokine (C-X-C) motif; CXCL, CXC ligand; CXCR, CXC receptor; CCL-3, chemokine (C-C motif) ligand 3; F15/30, treatment with 15/30 u m fisetin. {TM}

产品信息

701126

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2-5 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:20

产品规格

种属反应

Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

16H3L10

抗原

Recombinant protein corresponding to amino acids 32-100 of rat macrophage inflammatory protein 2

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2532403

产品详细信息

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Phosphate induces inflammation and exacerbates injury from cigarette smoke in the bronchial epithelium.

Scientific reports

Bollenbecker S,Heitman K,Czaya B,Easter M,Hirsch MJ,Vang S,Harris E,Helton ES,Barnes JW,Faul C,Krick S

701126 was used in Immunohistochemistry to demonstrate a novel pathway that potentially explains pathological kidney-lung crosstalk with phosphate as a key mediator.

Sat Mar 25 00:00:00 UTC 2023

Single-cell transcriptomics reveals distinct cell response between acute and chronic pulmonary infection of Pseudomonas aeruginosa.

MedComm

Hu X,Wu M,Ma T,Zhang Y,Zou C,Wang R,Zhang Y,Ren Y,Li Q,Liu H,Li H,Wang T,Sun X,Yang Y,Tang M,Li X,Li J,Gao X,Li T,Zhou X

701126 was used in Immunohistochemistry-immunofluorescence to provide a comprehensive and unbiased immune cellular landscape for respiratory pathogenesis in mice, enabling further understanding of immunologic mechanisms in infection and inflammatory diseases.

Thu Dec 01 00:00:00 UTC 2022

Single-cell transcriptomics reveals distinct cell response between acute and chronic pulmonary infection of Pseudomonas aeruginosa.

MedComm

Hu X,Wu M,Ma T,Zhang Y,Zou C,Wang R,Zhang Y,Ren Y,Li Q,Liu H,Li H,Wang T,Sun X,Yang Y,Tang M,Li X,Li J,Gao X,Li T,Zhou X

Thu Dec 01 00:00:00 UTC 2022

Associations of C-X-C motif chemokine ligands 1/2/8/13/14 with clinicopathological features and survival profile in patients with colorectal cancer.

Oncology letters

Luo X,Tai J,Zhao Y,Zhao P,Sun D,Wang L

701126 was used in Immunohistochemistry to assess the correlation of C-X-C motif chemokine ligand (CXCL)1, CXCL2, CXCL8, CXCL13 and CXCL14 with clinicopathological features and survival profile in patients with colorectal cancer (CRC).

Sat Oct 01 00:00:00 UTC 2022

Combination therapy of iPSC-derived conditioned medium with ceftriaxone alleviates bacteria-induced lung injury by targeting the NLRP3 inflammasome.

Journal of cellular physiology

Wann SR,Chi PL,Huang WC,Cheng CC,Chang YT

701126 was used in Immunohistochemistry (Paraffin), Western Blot to reveal the mechanisms underlying iPSC-CM-conferred anti-inflammatory activity in ALI through the attenuation of macrophage recruitment to the lung, thus inactivating NLRP3 inflammasomes in macrophages.

Tue Feb 01 00:00:00 UTC 2022

Monovalent antibody-conjugated lipid-polymer nanohybrids for active targeting to desmoglein 3 of keratinocytes to attenuate psoriasiform inflammation.

Theranostics

Lin ZC,Hwang TL,Huang TH,Tahara K,Trousil J,Fang JY

701126 was used in Immunohistochemistry to develop actively targeted nanocarriers loaded with the phosphodiesterase 4 inhibitor AN2728.

Mon Aug 16 00:00:00 UTC 2021

Monovalent antibody-conjugated lipid-polymer nanohybrids for active targeting to desmoglein 3 of keratinocytes to attenuate psoriasiform inflammation.

Theranostics

Lin ZC,Hwang TL,Huang TH,Tahara K,Trousil J,Fang JY

701126 was used in Immunohistochemistry to develop actively targeted nanocarriers loaded with the phosphodiesterase 4 inhibitor AN2728.

Mon Aug 16 00:00:00 UTC 2021

Lugdunin amplifies innate immune responses in the skin in synergy with host- and microbiota-derived factors.

Nature communications

Bitschar K,Sauer B,Focken J,Dehmer H,Moos S,Konnerth M,Schilling NA,Grond S,Kalbacher H,Kurschus FC,Götz F,Krismer B,Peschel A,Schittek B

701126 was used in Immunohistochemistry to indicate that lugdunin provides multi-level protection against S. aureus and may thus become a promising treatment option for S. aureus skin infections in the future.

Fri Jun 21 00:00:00 UTC 2019

Fisetin inhibits pristine-induced systemic lupus erythematosus in a murine model through CXCLs regulation.

International journal of molecular medicine

Xu SP,Li YS

Published figure using CXCL2 monoclonal antibody (Product # 701126) in Western Blot

Sat Dec 01 00:00:00 UTC 2018

生物信息学

蛋白别名: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 2; CC motif chemokine; CCmotif chemokine; CINC-3; CXC; CXC motif chemokine; CXCL; Cytokine-induced neutrophil chemoattractant 3; GRO b; gro beta; GRO β; GRObeta; GROβ; Macrophage inflammatory protein 2; MIP 2 a; MIP 2 alpha; MIP 2 α; MIP2; MIP2 a; MIP2 α; small inducible cytokine subfamily, member 2

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基因别名: CINC-2a; Cinc3; Cxcl2; Gro2; GROb; Mgsa-b; MIP-2; MIP-2a; Mip2; Scyb; Scyb2

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UniProt ID: (Mouse) P10889, (Rat) P30348

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Entrez Gene ID: (Mouse) 20310, (Rat) 114105

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功能

cytokine activity chemokine activity CXCR chemokine receptor binding chemokine

参与通路