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CYBA Antibody (MA5-18047) in ICC/IF
Immunofluorescence analysis of CYBA was performed using 70% confluent log phase PC-3, DU 145 and BJ cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CYBA Monoclonal Antibody (CS9) (Product # MA5-18047) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Produ... View More
Product Details
MA5-18047
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
CS9
Immunogen
Partially purified human neutrophil flavocytochrome b (heparin Ultrogellectin affinity purification in octyl glucoside)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2539430
Product Specific Information
This antibody is specific for human Cytochrome b245 light chain (aa 165-169, KKPSE).
This target has a predicted molecular weight of 21 kDa.
Target Information
Cytochrome b is comprised of a light chain (alpha) and a heavy chain (beta). This gene encodes the light, alpha subunit which has been proposed as a primary component of the microbicidal oxidase system of phagocytes. Mutations in this gene are associated with autosomal recessive chronic granulomatous disease (CGD), that is characterized by the failure of activated phagocytes to generate superoxide, which is important for the microbicidal activity of these cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytochrome b light chain; Cytochrome b(558) alpha chain; cytochrome b(558) alpha-subunit; cytochrome b, alpha polypeptide; Cytochrome b-245 light chain; cytochrome b-245, alpha polypeptide; Cytochrome b558 subunit alpha; flavocytochrome b-558 alpha polypeptide; Neutrophil cytochrome b 22 kDa polypeptide; p22 phagocyte B-cytochrome; p22-phox; p22phox; Superoxide-generating NADPH oxidase light chain subunit
Gene Aliases: CYBA; p22-PHOX
UniProt ID: (Human) P13498
Entrez Gene ID: (Human) 1535
response to reactive oxygen species
positive regulation of endothelial cell proliferation
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
negative regulation of glomerular filtration by angiotensin
superoxide metabolic process
inflammatory response
smooth muscle hypertrophy
cytochrome complex assembly
positive regulation of cell growth
response to nutrient levels
positive regulation of interleukin-6 production
positive regulation of tumor necrosis factor production
positive regulation of superoxide anion generation
positive regulation of toll-like receptor 2 signaling pathway
response to drug
superoxide anion generation
innate immune response
cell redox homeostasis
respiratory burst
vascular endothelial growth factor receptor signaling pathway
positive regulation of smooth muscle cell proliferation
hydrogen peroxide biosynthetic process
positive regulation of phagocytosis
regulation of release of sequestered calcium ion into cytosol
oxidation-reduction process
positive regulation of mucus secretion
response to interleukin-1
cellular response to amino acid stimulus
cellular response to mechanical stimulus
cellular response to glucose stimulus
cellular response to tumor necrosis factor
cellular response to organic cyclic compound
cellular response to gamma radiation
positive regulation of defense response to bacterium
positive regulation of reactive oxygen species biosynthetic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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