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CYP2A6 Antibody (PA1-342) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1) and Mouse Pancreas (Lane 2). The blots were probed with Anti-Cytochrome P450 2A6 Rabbit polyclonal Antibody (Product # PA1-342, 1:1000-1:5000 dilution dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 dilution). A 56 kDa band corresponding to Cytochrome P450 2A6 was observed in Hep G2 but not in Mouse Pancreas. Known quantity of protein samples were electrophore... View More
产品信息
PA1-342
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Purified, His-tagged, full length human P450 2A6 fusion protein.
偶联物
形式
Liquid
纯化类型
Ammonium sulfate precipitation
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2230297
产品详细信息
PA1-342 detects cytochrome P450 (P450) 2A6 from human, mouse and rat samples. This antibody detects, to a lesser extent, other P450 isoforms.
PA1-342 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~49 kDa protein representing P450 2A6 from rat liver extract.
The PA1-342 immunogen is purified, His-tagged, full length human P450 2A6 fusion protein.
靶标信息
This gene, CYP2A6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to hydroxylate coumarin, and also metabolizes nicotine, aflatoxin B1, nitrosamines, and some pharmaceuticals. Individuals with certain allelic variants are said to have a poor metabolizer phenotype, meaning they do not efficiently metabolize coumarin or nicotine. This gene is part of a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. The gene was formerly referred to as CYP2A3; however, it has been renamed CYP2A6.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 1,4-cineole 2-exo-monooxygenase; Coumarin 7-hydroxylase; CYP; CYPIIA6; Cytochrome P450 2A6; Cytochrome P450 IIA3; Cytochrome P450(I); cytochrome P450, family 2, subfamily A, polypeptide 6; cytochrome P450, subfamily IIA (phenobarbital-inducible), polypeptide 6; flavoprotein-linked monooxygenase; P450; P450 IIA3; P450(I); xenobiotic monooxygenase
基因别名: CPA6; CYP2A; CYP2A3; CYP2A6; CYPIIA6; P450C2A; P450PB
UniProt ID: (Human) P11509
Entrez Gene ID: (Human) 1548
iron ion binding
coumarin 7-hydroxylase activity
arachidonic acid epoxygenase activity
steroid hydroxylase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
enzyme binding
heme binding
