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CYP2R1 Antibody (PA5-101313) in ICC/IF
Immunocytochemistry analysis of CYP2R1 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with CYP2R1 polyclonal antibody (Product # PA5-101313) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-101313
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CYP2R1(Accession Q6VVX0), corresponding to amino acid residues C98-G148.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2850750
Product Specific Information
Antibody detects endogenous levels of total Cytochrome P450 2R1.
Target Information
CYP2R1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme is a microsomal vitamin D hydroxylase that converts vitamin D into the active ligand for the vitamin D receptor. A mutation in CYP2R1 gene has been associated with selective 25-hydroxyvitamin D deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cytochrome P450 2R1; cytochrome P450, 2r1; cytochrome P450, family 2, R1; cytochrome P450, family 2, subfamily R, polypeptide 1; Vitamin D 25-hydroxylase
Gene Aliases: CYP2R1
UniProt ID: (Human) Q6VVX0, (Mouse) Q6VVW9
Entrez Gene ID: (Human) 120227, (Mouse) 244209
iron ion binding
steroid hydroxylase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
heme binding
vitamin D3 25-hydroxylase activity
monooxygenase activity
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
oxygen binding
metal ion binding
cholestanetriol 26-monooxygenase activity
oxidoreductase
oxygenase
metabolite interconversion enzyme
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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