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CYP2R1 Antibody (PA5-101313) in ICC/IF
Immunocytochemistry analysis of CYP2R1 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with CYP2R1 polyclonal antibody (Product # PA5-101313) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-101313
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human CYP2R1(Accession Q6VVX0), corresponding to amino acid residues C98-G148.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2850750
产品详细信息
Antibody detects endogenous levels of total Cytochrome P450 2R1.
靶标信息
CYP2R1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme is a microsomal vitamin D hydroxylase that converts vitamin D into the active ligand for the vitamin D receptor. A mutation in CYP2R1 gene has been associated with selective 25-hydroxyvitamin D deficiency.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Cytochrome P450 2R1; cytochrome P450, 2r1; cytochrome P450, family 2, R1; cytochrome P450, family 2, subfamily R, polypeptide 1; Vitamin D 25-hydroxylase
基因别名: CYP2R1
UniProt ID: (Human) Q6VVX0, (Mouse) Q6VVW9
Entrez Gene ID: (Human) 120227, (Mouse) 244209
iron ion binding
steroid hydroxylase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
heme binding
vitamin D3 25-hydroxylase activity
monooxygenase activity
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
oxygen binding
metal ion binding
cholestanetriol 26-monooxygenase activity
oxidoreductase
oxygenase
metabolite interconversion enzyme
