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Invitrogen
CYP2R1 Polyclonal Antibody
FIGURE: 1 / 3
CYP2R1 Antibody (PA5-87927) in WB
Western blot analysis of CYP2R1 in extracts of various cell lines. Samples were incubated with CYP2R1 Polyclonal antibody (Product # PA5-87927) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Product Details
PA5-87927
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 252-501 of human CYP2R1 (NP_0787902)
Conjugate
Form
Liquid
Concentration
1.58 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2804510
Product Specific Information
Immunogen sequence: VVYDFLSRLI EKASVNRKPQ LPQHFVDAYL DEMDQGKNDP SSTFSKENLI FSVGELIIAG TETTTNVLRW AILFMALYPN IQGQVQKEID LIMGPNGKPS WDDKCKMPYT EAVLHEVLRF CNIVPLGIFH ATSEDAVVRG YSIPKGTTVI TNLYSVHFDE KYWRDPEVFH PERFLDSSGY FAKKEALVPF SLGRRHCLGE HLARMEMFLF FTALLQRFHL HFPHELVPDL KPRLGMTLQP QPYLICAERR; Positive Samples: Mouse testis, Rat liver; Cellular Location: Endoplasmic reticulum membrane, Microsome membrane, Peripheral membrane protein
Target Information
CYP2R1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme is a microsomal vitamin D hydroxylase that converts vitamin D into the active ligand for the vitamin D receptor. A mutation in CYP2R1 gene has been associated with selective 25-hydroxyvitamin D deficiency.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cytochrome P450 2R1; cytochrome P450, 2r1; Vitamin D 25-hydroxylase
Gene Aliases: Cyp2r1
UniProt ID: (Mouse) Q6VVW9
Entrez Gene ID: (Mouse) 244209, (Rat) 361631
monooxygenase activity
iron ion binding
steroid hydroxylase activity
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
oxygen binding
heme binding
vitamin D3 25-hydroxylase activity
metal ion binding
cholestanetriol 26-monooxygenase activity
molecular_function
oxidoreductase
oxygenase
metabolite interconversion enzyme
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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