Invitrogen

CYP3A4 Monoclonal Antibody (3H8)

CYP3A4 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 10

CYP3A4 Antibody (MA5-17064) in ICC/IF

Immunofluorescence analysis of HepG2 cells using CYP3A4 monoclonal antibody (Product # MA5-17064) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

CYP3A4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CYP3A4 Antibody in Flow Cytometry (Flow)

Product Image Gallery

产品信息

MA5-17064

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

查看3篇已发表文章 3篇已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:200-1:1,000

免疫细胞化学 (ICC/IF)

1:200-1:1,000

查看2篇已发表文章 2篇已发表文章

流式细胞分析 (Flow)

1:200-1:400

酶联免疫吸附实验 (ELISA)

1:10,000

产品规格

种属反应

Human

已发表种属

Human, Yeast

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

3H8

抗原

Purified recombinant fragment of human CYP3A4 expressed in E. Coli.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2538535

产品详细信息

MA5-17064 targets CYP3A4 in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human samples.

The MA5-17064 immunogen is purified recombinant fragment of human CYP3A4 expressed in E. Coli.

MA5-17064 detects CYP3A4 which has a predicted molecular weight of approximately 57.3kDa.

靶标信息

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The nuclear receptor THRB facilitates differentiation of human PSCs into more mature hepatocytes.

Cell stem cell

Ma H,de Zwaan E,Guo YE,Cejas P,Thiru P,van de Bunt M,Jeppesen JF,Syamala S,Dall'Agnese A,Abraham BJ,Fu D,Garrett-Engele C,Lee TI,Long HW,Griffith LG,Young RA,Jaenisch R

MA5-17064 was used in Immunohistochemistry-immunofluorescence to develop a 3D differentiation system and compared gene regulatory elements in human primary hepatocytes with those in hPSC-hepatocytes that were differentiated in 2D or 3D conditions by RNA-seq, ATAC-seq, and H3K27Ac ChIP-seq.

Thu May 05 00:00:00 UTC 2022

Commiphora myrrha (Nees) Engl. resin extracts induce phase-I cytochrome P450 2C8, 2C9, 2C19, and 3A4 isoenzyme expressions in human hepatocellular carcinoma (HepG2) cells.

Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society

Alehaideb Z,Alatar G,Nehdi A,Albaz A,Al-Eidi H,Almutairi M,Hawsa E,Alshuail N,Matou-Nasri S

MA5-17064 was used in Western Blot to study C. myrrha resin extracts on the expression of cytochrome P450 (CYP) drug-metabolizing isoenzyme in human hepatocellular carcinoma cell line HepG2.

Sat May 01 00:00:00 UTC 2021

Commiphora myrrha (Nees) Engl. resin extracts induce phase-I cytochrome P450 2C8, 2C9, 2C19, and 3A4 isoenzyme expressions in human hepatocellular carcinoma (HepG2) cells.

Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society

Alehaideb Z,Alatar G,Nehdi A,Albaz A,Al-Eidi H,Almutairi M,Hawsa E,Alshuail N,Matou-Nasri S

MA5-17064 was used in Western Blot to study C. myrrha resin extracts on the expression of cytochrome P450 (CYP) drug-metabolizing isoenzyme in human hepatocellular carcinoma cell line HepG2.

Sat May 01 00:00:00 UTC 2021

Overcoming bioprocess bottlenecks in the large-scale expansion of high-quality hiPSC aggregates in vertical-wheel stirred suspension bioreactors.

Stem cell research & therapy

Borys BS,Dang T,So T,Rohani L,Revay T,Walsh T,Thompson M,Argiropoulos B,Rancourt DE,Jung S,Hashimura Y,Lee B,Kallos MS

MA5-17064 was used in Immunohistochemistry to develop a scalable, single-cell inoculation protocol for the culture of hiPSCs as aggregates in vertical-wheel bioreactors, achieving over 30-fold expansion in 6 days without sacrificing cell quality.

Wed Jan 13 00:00:00 UTC 2021

Stirred suspension bioreactors maintain naïve pluripotency of human pluripotent stem cells.

Communications biology

Rohani L,Borys BS,Razian G,Naghsh P,Liu S,Johnson AA,Machiraju P,Holland H,Lewis IA,Groves RA,Toms D,Gordon PMK,Li JW,So T,Dang T,Kallos MS,Rancourt DE

MA5-17064 was used in Immunocytochemistry-immunoflourescence to investigate if conversion of hPSCs, from the conventional "primed" pluripotent state towards the "naïve" state prevalent in mPSCs, could be used to enhance hPSC production.

Mon Sep 07 00:00:00 UTC 2020

Stirred suspension bioreactors maintain naïve pluripotency of human pluripotent stem cells.

Communications biology

Rohani L,Borys BS,Razian G,Naghsh P,Liu S,Johnson AA,Machiraju P,Holland H,Lewis IA,Groves RA,Toms D,Gordon PMK,Li JW,So T,Dang T,Kallos MS,Rancourt DE

Mon Sep 07 00:00:00 UTC 2020

iTAG-RNA Isolates Cell-Specific Transcriptional Responses to Environmental Stimuli and Identifies an RNA-Based Endocrine Axis.

Cell reports

Darr J,Tomar A,Lassi M,Gerlini R,Berti L,Hering A,Scheid F,Hrabě de Angelis M,Witting M,Teperino R

MA5-17064 was used in Western Blotting to demonstrate in vivo transfer of RNAs between tissues and highlight its implications for endocrine signaling and homeostasis.

Tue Mar 03 00:00:00 UTC 2020

Improving Drug Discovery by Nucleic Acid Delivery in Engineered Human Microlivers.

Cell metabolism

Mancio-Silva L,Fleming HE,Miller AB,Milstein S,Liebow A,Haslett P,Sepp-Lorenzino L,Bhatia SN

Published figure using CYP3A4 monoclonal antibody (Product # MA5-17064) in Immunocytochemistry

Tue Mar 05 00:00:00 UTC 2019

An in vitro system for measuring genotoxicity mediated by human CYP3A4 in Saccharomyces cerevisiae.

Environmental and molecular mutagenesis

Fasullo M,Freedland J,St John N,Cera C,Egner P,Hartog M,Ding X

MA5-17064 was used in Western Blot to confirm the ability of human CYP3A4 to mediate the genotoxicity of AFB1 , and illustrate the usefulness of the CYP3A4-expressing, DNA-repair mutant yeast strain for screening other chemical compounds that are CYP3A4 substrates, for potential genotoxicity.

Mon May 01 00:00:00 UTC 2017

生物信息学

蛋白别名: 1,4-cineole 2-exo-monooxygenase; 1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; Albendazole monooxygenase (sulfoxide-forming); Albendazole sulfoxidase; Cholesterol 25-hydroxylase; CYP; CYPIIIA3; CYPIIIA4; Cytochrome P450 3A3; Cytochrome P450 3A4; Cytochrome P450 HLp; Cytochrome P450 NF-25; cytochrome P450, family 3, subfamily A, polypeptide 4; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4; Cytochrome P450-PCN1; glucocorticoid-inducible P450; MGC126680; Nifedipine oxidase; P450; P450-III, steroid inducible; P450-PCN1; Quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase

查看更多收起

基因别名: CP33; CP34; CYP3A; CYP3A3; CYP3A4; CYPIIIA3; CYPIIIA4; HLP; NF-25; P450C3; P450PCN1

查看更多收起

UniProt ID: (Human) P08684

查看更多收起

Entrez Gene ID: (Human) 1576

查看更多收起

功能