Invitrogen

CaMKII delta Polyclonal Antibody

CaMKII delta Antibody in Immunocytochemistry (ICC/IF)

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CaMKII delta Antibody (PA5-22168) in ICC/IF

Immunofluorescence analysis of CamKII delta was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CaMKII delta Polyclonal Antibody (Product # PA5-22168) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsiu... View More

CaMKII delta Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CaMKII delta Antibody in Western Blot (WB)

CaMKII delta Antibody in Immunoprecipitation (IP)

Product Image Gallery

产品信息

PA5-22168

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:50,000

查看8篇已发表文章 8篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

免疫沉淀 (IP)

1:100-1:500

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Rabbit, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein fragment contain a sequence corresponding to a region within amino acids 249 and 445 (Q13557) of CaMKII delta

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.66 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_11153337

产品详细信息

Recommended positive controls: 293T, A431, HeLa, HepG2, A549, H1299, HCT116, mouse brain, rat brain.

Predicted reactivity: Mouse (100%), Rat (80%), Xenopus laevis (94%), Pig (100%), Rabbit (99%), Chicken (98%), Bovine (92%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

CaMKII delta belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. is a delta chain. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Distinct isoforms of this chain have different expression patterns.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

CaMKII and reactive oxygen species contribute to early reperfusion arrhythmias, but oxidation of CaMKIIδ at methionines 281/282 is not a determining factor.

Journal of molecular and cellular cardiology

Haugsten Hansen M,Sadredini M,Hasic A,Anderson ME,Sjaastad I,Korseberg Stokke M

PA5-22168 was used in Western Blotting to investigate the role of oxidized-CaMKIIδ (Ox-CaMKIIδ) in early ischemia-reperfusion arrhythmias (IRA).

Wed Feb 01 00:00:00 UTC 2023

Detrimental proarrhythmogenic interaction of Ca²⁺/calmodulin-dependent protein kinase II and Na_V1.8 in heart failure.

Nature communications

Bengel P,Dybkova N,Tirilomis P,Ahmad S,Hartmann N,A Mohamed B,Krekeler MC,Maurer W,Pabel S,Trum M,Mustroph J,Gummert J,Milting H,Wagner S,Ljubojevic-Holzer S,Toischer K,Maier LS,Hasenfuss G,Streckfuss-Bömeke K,Sossalla S

PA5-22168 was used in Western Blot to elucidate the role of the sodium channel isoform NaV1.8 for CaMKIIδc-dependent proarrhythmia.

Mon Nov 15 00:00:00 UTC 2021

Detrimental proarrhythmogenic interaction of Ca²⁺/calmodulin-dependent protein kinase II and Na_V1.8 in heart failure.

Nature communications

PA5-22168 was used in Western Blot to elucidate the role of the sodium channel isoform NaV1.8 for CaMKIIδc-dependent proarrhythmia.

Mon Nov 15 00:00:00 UTC 2021

Detrimental proarrhythmogenic interaction of Ca²⁺/calmodulin-dependent protein kinase II and Na_V1.8 in heart failure.

Nature communications

PA5-22168 was used in Western Blot to elucidate the role of the sodium channel isoform NaV1.8 for CaMKIIδc-dependent proarrhythmia.

Mon Nov 15 00:00:00 UTC 2021

The oxidation-resistant CaMKII-MM281/282VV mutation does not prevent arrhythmias in CPVT1.

Physiological reports

Sadredini M,Manotheepan R,Lehnart SE,Anderson ME,Sjaastad I,Stokke MK

PA5-22168 was used in Western Blotting to test whether an oxidation-resistant form of CaMKII protects mice carrying the CPVT1-causative mutation RyR2-R2474S (RyR2-RS) against arrhythmias.

Wed Sep 01 00:00:00 UTC 2021

Gating of hippocampal rhythms and memory by synaptic plasticity in inhibitory interneurons.

Neuron

He X,Li J,Zhou G,Yang J,McKenzie S,Li Y,Li W,Yu J,Wang Y,Qu J,Wu Z,Hu H,Duan S,Ma H

PA5-22168 was used in Western Blotting to suggest that E→I synaptic plasticity, exemplified by LTPE→I, plays a gatekeeping role in tuning experience-dependent brain rhythms and mnemonic function.

Wed Mar 17 00:00:00 UTC 2021

Long-term effects of empagliflozin on excitation-contraction-coupling in human induced pluripotent stem cell cardiomyocytes.

Journal of molecular medicine (Berlin, Germany)

Pabel S,Reetz F,Dybkova N,Shomroni O,Salinas G,Mustroph J,Hammer KP,Hasenfuss G,Hamdani N,Maier LS,Streckfuss-Bömeke K,Sossalla S

PA5-22168 was used in Western Blotting to investigate the long-term effects (up to 2 months) of empagliflozin on excitation-contraction (EC)-coupling in human cardiomyocytes derived from induced pluripotent stem cells (iPSC-CM) in a blinded manner.

Tue Dec 01 00:00:00 UTC 2020

Long-term effects of empagliflozin on excitation-contraction-coupling in human induced pluripotent stem cell cardiomyocytes.

Journal of molecular medicine (Berlin, Germany)

Pabel S,Reetz F,Dybkova N,Shomroni O,Salinas G,Mustroph J,Hammer KP,Hasenfuss G,Hamdani N,Maier LS,Streckfuss-Bömeke K,Sossalla S

Tue Dec 01 00:00:00 UTC 2020

Inhibition of calcium/calmodulin-dependent kinase II restores contraction and relaxation in isolated cardiac muscle from type 2 diabetic rats.

Cardiovascular diabetology

Daniels LJ,Wallace RS,Nicholson OM,Wilson GA,McDonald FJ,Jones PP,Baldi JC,Lamberts RR,Erickson JR

Published figure using CaMKII delta polyclonal antibody (Product # PA5-22168) in Western Blot

Thu Jun 14 00:00:00 UTC 2018

Arrhythmias precede cardiomyopathy and remodeling of Ca²⁺ handling proteins in a novel model of long QT syndrome.

Journal of molecular and cellular cardiology

Montnach J,Chizelle FF,Belbachir N,Castro C,Li L,Loussouarn G,Toumaniantz G,Carcouët A,Meinzinger AJ,Shmerling D,Benitah JP,Gómez AM,Charpentier F,Baró I

PA5-22168 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to show that the Scn5a+/ΔQKP mouse model recapitulates the clinical phenotype of mutation carriers and provides new and unexpected insights into the pathological development of the disease in patients carrying the QKP1507-1509 deletion.

Mon Oct 01 00:00:00 UTC 2018

Beta-Adrenoceptor Stimulation Reveals Ca2+ Waves and Sarcoplasmic Reticulum Ca2+ Depletion in Left Ventricular Cardiomyocytes from Post-Infarction Rats with and without Heart Failure.

PloS one

Sadredini M,Danielsen TK,Aronsen JM,Manotheepan R,Hougen K,Sjaastad I,Stokke MK

Published figure using CaMKII delta polyclonal antibody (Product # PA5-22168) in Western Blot

Fri Sep 09 00:00:00 UTC 2016

生物信息学

蛋白别名: Ca++/calmodulin-dependent protein kinase 2 delta subunit; Ca++/calmodulin-dependent protein kinase II delta subunit; Ca++/calmodulin-dependent protein kinase II, delta subunit; calcium/calmodulin-dependent protein kinase (CaM kinase) II delta; calcium/calmodulin-dependent protein kinase II delta; calcium/calmodulin-dependent protein kinase type II delta chain; Calcium/calmodulin-dependent protein kinase type II subunit delta; calmodulin-dependent protein kinase II-delta dash; CaM kinase II delta subunit; CaM kinase II subunit delta; CaM-kinase II delta chain; CAM2; CaMK-II delta subunit; CaMK-II subunit delta; CAMK2B; CAMK2G; CAMKB; CAMKG

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基因别名: 2810011D23Rik; 8030469K03Rik; [d]-CaMKII; CaMK II; CAMK1; Camk2; CAMK2D; CAMKD; Camki; Kiaa4163; RATCAMKI