Invitrogen

CaV3.1 Monoclonal Antibody (N178A/9), APC

CaV3.1 Antibody in Immunocytochemistry (ICC/IF)

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CaV3.1 Antibody (MA5-45543) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with Cav3.1 monoclonal antibody (Product # MA5-45543) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X. (A) DAPI (blue) nuclear st... View More

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产品信息

MA5-45543

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化 (IHC)

Assay-dependent

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

N178A/9

抗原

Fusion protein amino acids 2052-2172 (cytoplasmic C-terminus) of mouse Cav3.1

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA

内含物

no preservative

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_2931997

产品详细信息

1 µg/mL of MA5-45543 was sufficient for detection of Cav3.1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately <200kDa. Does not cross-react with Cav3.2.

This antibody was formerly sold as clone S178A-9.

靶标信息

Voltage-dependent Ca2+ channels mediate Ca2+ entry into excitable cells in response to membrane depolarization, and they are involved in a variety of Ca2+-dependent processes, including muscle contraction, hormone or neurotransmitter release and gene expression. Calcium channels are highly diverse, multimeric complexes composed of an alpha-1 subunit, an intracellular beta subunit, a disulfide linked alpha-2/delta subunit and a transmembrane gamma subunit. Ca2+ currents are characterized on the basis of their biophysical and pharmacologic properties and include L-, N-, T-, P-, Q-, and R- types. T-type Ca2+ currents are activated and inactivated more rapidly and at more negative membrane potentials than other Ca2+ current types. T-type Ca2+ channels enhance odor sensitivity by lowering the threshold of spike generation in olfactory receptor cells (ORCs).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: calcium channel, voltage-dependent, T type, alpha 1G subunit; CaV T1; Cav3 1; cav3 1c; Cav3.1c; MGC117234; NBR13; voltage-dependent calcium channel alpha 1G subunit, isoform 11; voltage-dependent T-type calcium channel alpha 1G subunit; Voltage-dependent T-type calcium channel subunit alpha-1G; Voltage-gated calcium channel subunit alpha Cav3.1

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基因别名: [a]1G; a1G; alpha-1G; Ca(V)T.1; CACNA1G; Cav3.1; Cav3.1d; KIAA1123; mKIAA1123; NBR13; SCA42

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UniProt ID: (Human) O43497

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Entrez Gene ID: (Human) 8913, (Rat) 29717, (Mouse) 12291

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功能

low voltage-gated calcium channel activity voltage-gated calcium channel activity involved in AV node cell action potential voltage-gated calcium channel activity involved SA node cell action potential scaffold protein binding voltage-gated calcium channel activity protein binding voltage-gated ion channel

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