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FIGURE: 1 / 4
Calcineurin B Antibody (PA5-141056) in ICC/IF
Immunocytochemistry analysis of Calcineurin B in SiHa cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 &(8)451;, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Samples were incubated in Calcineurin B Polyclonal antibody (Product # PA5-141056) using a dilution of 1:200 in 2% negative goat serum o... View More
Product Details
PA5-141056
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within Human Calcineurin B aa 1-170/170.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 40% glycerol, 0.1% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2932508
Target Information
Calcineurin is a Ser/Thr, calcium and calmodulin-dependent protein phosphatase that plays an essential role in the T cell activation pathway. Calcineurin (also referred to as protein phosphatase 2B) is composed of two subunits; calcineurin A (CnA), a 60 kDa catalytic subunit which is highly homologous to protein phosphatases 1 and 2A; and a 19 kDa regulatory subunit, calcineurin B (CnB) which, like calmodulin, contains four EF-hand calcium binding sites. Calcineurin-mediated dephosphorylation of the nuclear factor of activated T-cells (NF-AT) is essential for NF-AT activation, nuclear translocation, and early gene expression in T-cells. The immunosuppressive drugs, cyclosporin A and FK506, when bound to the immunophilins, cyclophilin and FKBP respectively, inhibit dephosphorylation and activation of NF-AT by calcineurin. This inhibition is caused by the immunosuppressant/immunophilin complex simultaneously binding to both CnA and CnB which does not inhibit the phosphatase activity of CnA, but rather blocks access of the substrate to the catalytic domain of CnA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ALNB1; calcineurin B, type I (19kDa); Calcineurin subunit B type 1; PP2B beta 1; protein phospatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I); protein phospatase 3, regulatory subunit B, alpha isoform,type 1; Protein phosphatase 2B regulatory subunit 1; protein phosphatase 2B regulatory subunit B alpha; protein phosphatase 3 (formerly 2B), regulatory subunit B (19kD), alpha isoform (calcineurin B, type I); protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I); protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform; protein phosphatase 3 regulatory subunit B alpha; Protein phosphatase 3 regulatory subunit B alpha isoform 1; protein phosphatase 3, regulatory subunit B, alpha; protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I)
Gene Aliases: CALNB1; CaNB1; CNA2; CNB; CNB1; MCIP1; PPP3R1
UniProt ID: (Human) P63098, (Mouse) Q63810, (Rat) P63100
Entrez Gene ID: (Human) 5534, (Mouse) 19058, (Rat) 29748
protein dephosphorylation
Wnt signaling pathway, calcium modulating pathway
calcineurin-NFAT signaling cascade
Fc-epsilon receptor signaling pathway
positive regulation of transcription from RNA polymerase II promoter
positive regulation of NFAT protein import into nucleus
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
patterning of blood vessels
epithelial to mesenchymal transition
heart development
Schwann cell development
myelination in peripheral nervous system
protein localization to nucleus
NFAT protein import into nucleus
lung epithelial cell differentiation
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