Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 3
Calcineurin B Antibody (MA5-49305) in IHC (P)
Immunohistochemistry analysis of Calcineurin B in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. Samples were incubated with Calcineurin B Monoclonal antibody (Product # MA5-49305) using a dilution of 1 µg/mL overnight at 4°C. Peroxidase Conjugated Goat Ant... View More
产品信息
MA5-49305
产品规格
种属反应
Bovine,
Human,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
CN-A1
抗原
Bovine brain calcineurin.
偶联物
形式
Lyophilized
浓度
Conc. Not Determined
保存液
ascites, 1.2% sodium acetate
内含物
0.01mg sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_3074963
产品详细信息
Adding 1 ml of PBS buffer will yield a concentration of 100 µg/mL.
靶标信息
Calcineurin is a Ser/Thr, calcium and calmodulin-dependent protein phosphatase that plays an essential role in the T cell activation pathway. Calcineurin (also referred to as protein phosphatase 2B) is composed of two subunits; calcineurin A (CnA), a 60 kDa catalytic subunit which is highly homologous to protein phosphatases 1 and 2A; and a 19 kDa regulatory subunit, calcineurin B (CnB) which, like calmodulin, contains four EF-hand calcium binding sites. Calcineurin-mediated dephosphorylation of the nuclear factor of activated T-cells (NF-AT) is essential for NF-AT activation, nuclear translocation, and early gene expression in T-cells. The immunosuppressive drugs, cyclosporin A and FK506, when bound to the immunophilins, cyclophilin and FKBP respectively, inhibit dephosphorylation and activation of NF-AT by calcineurin. This inhibition is caused by the immunosuppressant/immunophilin complex simultaneously binding to both CnA and CnB which does not inhibit the phosphatase activity of CnA, but rather blocks access of the substrate to the catalytic domain of CnA.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: ALNB1; calcineurin B; calcineurin B, type I (19kDa); Calcineurin subunit B type 1; protein phospatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I); protein phospatase 3, regulatory subunit B, alpha isoform,type 1; Protein phosphatase 2B regulatory subunit 1; protein phosphatase 2B regulatory subunit B alpha; protein phosphatase 3 (formerly 2B), regulatory subunit B (19kD), alpha isoform (calcineurin B, type I); protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I); protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform; Protein phosphatase 3 regulatory subunit B alpha isoform 1; protein phosphatase 3, regulatory subunit B, alpha; protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I)
基因别名: CALNB1; CNA2; CNB; CNB1; PPP3R1
UniProt ID: (Human) P63098, (Bovine) P63099, (Rat) P63100
Entrez Gene ID: (Human) 5534, (Bovine) 282321, (Rat) 29748
protein dephosphorylation
Wnt signaling pathway, calcium modulating pathway
calcineurin-NFAT signaling cascade
Fc-epsilon receptor signaling pathway
positive regulation of transcription from RNA polymerase II promoter
positive regulation of NFAT protein import into nucleus
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
patterning of blood vessels
epithelial to mesenchymal transition
heart development
Schwann cell development
myelination in peripheral nervous system
protein localization to nucleus
NFAT protein import into nucleus
lung epithelial cell differentiation
protein import into nucleus
regulation of postsynaptic neurotransmitter receptor internalization
postsynaptic modulation of chemical synaptic transmission
