Invitrogen

Calnexin Polyclonal Antibody

Calnexin Antibody in Immunocytochemistry (ICC/IF)

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Calnexin Antibody (PA5-34804) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of Calnexin was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: Calnexin Polyclonal Antibody (Product # PA5-34804) diluted at 1:200. Blue: Hoechst 33342 staining.

Calnexin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-34804

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:10,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 59 and 304 of Calnexin (Uniprot ID#P27824)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.53 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 10% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2552156

产品详细信息

Recommended positive controls: 293T, A431, HeLa, HepG2, mouse liver, PC-12, Rat2.

Predicted reactivity: Mouse (97%), Rat (98%), Zebrafish (82%), Xenopus laevis (86%), Dog (98%), Pig (96%), Chicken (92%), Bovine (97%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Calnexin, also referred to as IP90, p88 and p90, is an approximately 90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatibility complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP78 and GRP94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids).

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。