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Calpain 3 Antibody (PA5-26818) in IHC (P)
Immunohistochemistry analysis of Calpain 3 in formalin fixed and paraffin embedded skeletal muscle. Samples were incubated with Calpain 3 polyclonal antibody (Product # PA5-26818) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Product Details
PA5-26818
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 603-632 amino acids from the C-terminal region of human CAPN3
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Ammonium sulfate precipitation, Size-exclusion - Dialysis
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2544318
Product Specific Information
This antibody is predicted to react with non-human primate based on sequence homology.
Target Information
Calpain, an intracellular calcium-dependent protease that cleaves cytoskeletal and submembranous proteins, plays a role in cytoskeletal reorganization and muscle protein degradation. Calpain is a heterodimer composed of a small regulatory subunit and one of three large catalytic subunits, designated calpain 1, calpain 2 and calpain 3. Calpain 3 (calpain p94) is a muscle-preferred calcium activated neutral protease that localizes to the nucleus. Mutations involving the calpain 3 gene are associated with limb-girdle muscle dystrophy type 2A, a form of autosomal recessive and progressive neuromuscular diso4. Calpastatin regulates calpain by inhibiting both the proteolytic activity of calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Calcium-activated neutral proteinase 3; calpain 3, (p94); Calpain L3; Calpain p94; calpain p94, large [catalytic] subunit; calpain, large polypeptide L3; Calpain-3; CANP 3; CAPN 3; LGMD 2A; MGC10767; MGC11121; MGC14344; MGC4403; Muscle-specific calcium-activated neutral protease 3; muscle-specific calcium-activated neutral protease 3 large subunit; nCL-1; New calpain 1
Gene Aliases: CANP3; CANPL3; CAPN3; LGMD2; LGMD2A; nCL-1; NCL1; p94
UniProt ID: (Human) P20807
Entrez Gene ID: (Human) 825
catalytic activity
calcium-dependent cysteine-type endopeptidase activity
signal transducer activity
calcium ion binding
protein binding
peptidase activity
cysteine-type peptidase activity
structural constituent of muscle
sodium ion binding
titin binding
protein complex scaffold
ligase regulator activity
cysteine protease
protein complex assembly
proteolysis
apoptotic process
signal transduction
muscle organ development
positive regulation of satellite cell activation involved in skeletal muscle regeneration
response to muscle activity
myofibril assembly
negative regulation of protein sumoylation
negative regulation of apoptotic process
regulation of I-kappaB kinase/NF-kappaB signaling
sarcomere organization
regulation of myoblast differentiation
positive regulation of proteolysis
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
muscle cell cellular homeostasis
regulation of catalytic activity
positive regulation of NF-kappaB transcription factor activity
positive regulation of release of sequestered calcium ion into cytosol
response to calcium ion
muscle structure development
G1 to G0 transition involved in cell differentiation
cellular response to calcium ion
cellular response to salt stress
protein localization to membrane
self proteolysis
negative regulation of skeletal muscle cell differentiation
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