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Calsequestrin Antibody (PA5-85406) in WB
Western blot was performed using Anti-Calsequestrin Polyclonal Antibody (Product # PA5-85406) and a 45 kDa band corresponding to Calsequestrin-1 was observed across tissues tested . Tissue extracts (30 µg lysate) of Mouse Skeletal Muscle (Lane 1), Rat Skeletal Muscle (Lane 2), Rat Heart (Lane 3), Mouse Liver (Lane 4), Rat Liver (Lane 5), Mouse Kidney (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® ... View More
产品信息
PA5-85406
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein encompassing a sequence within the center region of human Calsequestrin 1.
偶联物
形式
Liquid
浓度
0.79 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol, 1% BSA
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2792548
产品详细信息
Keep as concentrated solution.
Predicted reactivity: Mouse (96%), Rat (96%), Pig (96%), Rabbit (96%), Bovine (94%).
Positive Control: HepG2, mouse muscle, rat muscle.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
The sarcoplasmic reticulum (SR) is, in part, responsible for maintaining the level of intracellular calcium in cardiac and skeletal muscle by storing and releasing calcium. Several intralumenal SR calcium binding proteins have been identified, the most prominent of these is calsequestrin. Calsequstrin is a calcium binding protein known to sequester calcium accumulated in the sarcoplasmic reticulum of muscle cells during relaxation and is found discretely localized to the junctional and corbular (terminal cisternae) SR. Calsequestrin functions to localize calcium near the junctional face of the terminal cisternae from which calcium can be released into the cytosol via the ryanodine receptor. This protein is highly acidic and has a large capacity and moderate to low affinity for calcium.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Aspartactin; calmitin; Calmitine; calsequestrin 1 (fast-twitch, skeletal muscle); Calsequestrin, skeletal muscle isoform; Calsequestrin-1; Laminin-binding protein
基因别名: CASQ; CASQ1; CSQ; CSQ-1; CSQ1; PDIB1; sCSQ; VMCQA
UniProt ID: (Human) P31415, (Rat) P19633, (Mouse) O09165
Entrez Gene ID: (Human) 844, (Rat) 686019, (Mouse) 12372
endoplasmic reticulum organization
skeletal muscle tissue development
response to heat
response to organic substance
regulation of skeletal muscle contraction by regulation of release of sequestered calcium ion
response to denervation involved in regulation of muscle adaptation
ion transmembrane transport
sarcomere organization
protein polymerization
regulation of cardiac conduction
regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
response to muscle inactivity
regulation of sequestering of calcium ion
regulation of muscle contraction
