Invitrogen

Cannabinoid Receptor 1 Polyclonal Antibody

Cannabinoid Receptor 1 Antibody in Immunohistochemistry (IHC)

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Cannabinoid Receptor 1 Antibody (PA1-745) in IHC

Immunohistochemical staining of the cannibinoid receptor 1 in transverse spinal cord sections of E6 chick embryo using Product # PA1-745.

Cannabinoid Receptor 1 Antibody in Western Blot (WB)

Cannabinoid Receptor 1 Antibody in Immunohistochemistry, Proximity Ligation Assay (PLA) (IHC, PLA)

Cannabinoid Receptor 1 Antibody in Flow Cytometry (Flow)

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Product Details

PA1-745

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:1,000

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

View 3 publications 3 publications

in situ PLA (PLA)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Amphibian, Chicken, Fish, Human, Mouse, Non-human primate, Rat

Published species

Cynomolgus monkey, Ferret, Human, Mouse, Rabbit, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A fusion protein containing the first 77 amino acid residues from rat CB1.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2082752

Product Specific Information

PA1-745 detects cannabinoid receptor 1 (CB1) from human, rat, monkey, mouse, chicken, salamander and goldfish tissues. This antibody detects a few non-specific bands in NIH-3T3 and HT-29 cell lysates.

PA1-745 has been successfully used in Western blot, FACS, immunohistochemistry, and immunocytochemistry procedures. By Western blot, this antibody detects a ~60 kDa protein representing CB1 and less intense bands of ~23 kDa, ~72 kDa, and ~180 kDa from rat brain homogenate. Immunocytochemical staining of CB1 in living or fixed cultured hippocampal neurons with PA1-745 results in intense, punctate staining along processes, with little staining over cell bodies.

PA1-745 antigen is a fusion protein containing the first 77 amino acid residues of the rat CB1 receptor.

Target Information

This gene encodes one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protein coupled receptor family, which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Identification of BiP as a CB₁ Receptor-Interacting Protein That Fine-Tunes Cannabinoid Signaling in the Mouse Brain.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Costas-Insua C,Moreno E,Maroto IB,Ruiz-Calvo A,Bajo-Grañeras R,Martín-Gutiérrez D,Diez-Alarcia R,Vilaró MT,Cortés R,García-Font N,Martín R,Espina M,Botta J,Ginés S,McCormick PJ,Sánchez-Prieto J,Galve-Roperh I,Mengod G,Urigüen L,Marsicano G,Bellocchio L,Canela EI,Casadó V,Rodríguez-Crespo I,Guzmán M

PA1-745 was used in Proximity Ligation Assay (PLA) to show BiP as a potential Type 1 cannabinoid receptorCB1R-interacting protein by using a yeast two-hybrid-based high-throughput screening.

Wed Sep 22 00:00:00 UTC 2021

Identification of BiP as a CB₁ Receptor-Interacting Protein That Fine-Tunes Cannabinoid Signaling in the Mouse Brain.

The Journal of neuroscience : the official journal of the Society for Neuroscience

PA1-745 was used in Proximity Ligation Assay (PLA) to show BiP as a potential Type 1 cannabinoid receptorCB1R-interacting protein by using a yeast two-hybrid-based high-throughput screening.

Wed Sep 22 00:00:00 UTC 2021

Methamphetamine Blocks Adenosine A_2A Receptor Activation via Sigma 1 and Cannabinoid CB₁ Receptors.

International journal of molecular sciences

Casanovas M,Reyes-Resina I,Lillo A,Lillo J,López-Arnau R,Camarasa J,Escubedo E,Navarro G,Franco R

PA1-745 was used in Proximity Ligation Assay (PLA) to investigate the impact of methamphetamine on adenosine A2A receptor (A2AR) and cannabinoid CB1 receptor (CB1R) complexes.

Tue Mar 09 00:00:00 UTC 2021

Expression of cannabinoid CB₁ R-GPR55 heteromers in neuronal subtypes of the Macaca fascicularis striatum.

Annals of the New York Academy of Sciences

Martínez-Pinilla E,Rico AJ,Rivas-Santisteban R,Lillo J,Roda E,Navarro G,Franco R,Lanciego JL

PA1-745 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to characterize cannabinoid CB1 R-GPR55 heteromers (CB1 R/GPR55Hets) in the basal ganglia input nuclei of nonhuman primates, Macaca fascicularis, both in projection neurons and interneurons, by the in situ proximity ligation assay.

Tue Sep 01 00:00:00 UTC 2020

Expression of cannabinoid CB₁ R-GPR55 heteromers in neuronal subtypes of the Macaca fascicularis striatum.

Annals of the New York Academy of Sciences

Martínez-Pinilla E,Rico AJ,Rivas-Santisteban R,Lillo J,Roda E,Navarro G,Franco R,Lanciego JL

Tue Sep 01 00:00:00 UTC 2020

Expression of cannabinoid CB₁ R-GPR55 heteromers in neuronal subtypes of the Macaca fascicularis striatum.

Annals of the New York Academy of Sciences

Martínez-Pinilla E,Rico AJ,Rivas-Santisteban R,Lillo J,Roda E,Navarro G,Franco R,Lanciego JL

Tue Sep 01 00:00:00 UTC 2020

Chemical Tools for Studying Lipid-Binding Class A G Protein-Coupled Receptors.

Pharmacological reviews

Cooper A,Singh S,Hook S,Tyndall JDA,Vernall AJ

PA1-745 was used in Flow cytometry/Cell sorting to review the radioligands, fluorescent ligands, covalent ligands, and antibodies that have been used to studying lipid-binding receptors.

Sat Jul 01 00:00:00 UTC 2017

Sex-dependent influence of chronic mild stress (CMS) on voluntary alcohol consumption; study of neurobiological consequences.

Pharmacology, biochemistry, and behavior

Marco EM,Ballesta JA,Irala C,Hernández MD,Serrano ME,Mela V,López-Gallardo M,Viveros MP

PA1745 was used in western blot to study voluntary alcohol consumption after chronic mild stress in Wistar rats

Sun Jan 01 00:00:00 UTC 2017

Tumor-promoting effects of cannabinoid receptor type 1 in human melanoma cells.

Toxicology in vitro : an international journal published in association with BIBRA

Carpi S,Fogli S,Polini B,Montagnani V,Podestà A,Breschi MC,Romanini A,Stecca B,Nieri P

PA1745 was used in western blot to investigate whether cannabinoid-type 1 receptor functions as tumor-promoting or -suppressing signal in human cutaneous melanoma

Sat Apr 01 00:00:00 UTC 2017

Striatal adenosine-cannabinoid receptor interactions in rats over-expressing adenosine A2A receptors.

Journal of neurochemistry

Chiodi V,Ferrante A,Ferraro L,Potenza RL,Armida M,Beggiato S,Pèzzola A,Bader M,Fuxe K,Popoli P,Domenici MR

PA1-745 was used in western blot to study the interactions in rats that over-express adenosine A2A receptors and striatal adenosine-cannabinoid receptors

Tue Mar 01 00:00:00 UTC 2016

Microarray analysis of transcripts with elevated expressions in the rat medial or lateral habenula suggest fast GABAergic excitation in the medial habenula and habenular involvement in the regulation of feeding and energy balance.

Brain structure & function

Wagner F,Bernard R,Derst C,French L,Veh RW

PA1745 was used in immunohistochemistry to determine if medial or lateral habenula or their subnuclei display field-characteristic gene products

Thu Dec 01 00:00:00 UTC 2016

Cannabinoid Receptors Are Overexpressed in CLL but of Limited Potential for Therapeutic Exploitation.

PloS one

Freund P,Porpaczy EA,Le T,Gruber M,Pausz C,Staber P,Jäger U,Vanura K

PA1-745 was used in western blot to study overexpression in cannabinoid receptors of CLL but a limited potential for therapeutic exploitation

Tue Jul 18 00:00:00 UTC 2017

Effects of pro-inflammatory cytokines on cannabinoid CB1 and CB2 receptors in immune cells.

Acta physiologica (Oxford, England)

Jean-Gilles L,Braitch M,Latif ML,Aram J,Fahey AJ,Edwards LJ,Robins RA,Tanasescu R,Tighe PJ,Gran B,Showe LC,Alexander SP,Chapman V,Kendall DA,Constantinescu CS

PA1-745 was used in flow cytometry and western blot to investigate the effects of proinflammatory cytokines on cannabinoid receptor expression and function

Fri May 01 00:00:00 UTC 2015

Effects of pro-inflammatory cytokines on cannabinoid CB1 and CB2 receptors in immune cells.

Acta physiologica (Oxford, England)

Jean-Gilles L,Braitch M,Latif ML,Aram J,Fahey AJ,Edwards LJ,Robins RA,Tanasescu R,Tighe PJ,Gran B,Showe LC,Alexander SP,Chapman V,Kendall DA,Constantinescu CS

PA1-745 was used in flow cytometry and western blot to investigate the effects of proinflammatory cytokines on cannabinoid receptor expression and function

Fri May 01 00:00:00 UTC 2015

Effects of palmitoylation of Cys(415) in helix 8 of the CB(1) cannabinoid receptor on membrane localization and signalling.

British journal of pharmacology

Oddi S,Dainese E,Sandiford S,Fezza F,Lanuti M,Chiurchiù V,Totaro A,Catanzaro G,Barcaroli D,De Laurenzi V,Centonze D,Mukhopadhyay S,Selent J,Howlett AC,Maccarrone M

PA1-745 was used in immunocytochemistry to investigate the role of CB(1) receptor palmitoylation in membrane localization and signalling

Sun Apr 01 00:00:00 UTC 2012

Unbalance of CB1 receptors expressed in GABAergic and glutamatergic neurons in a transgenic mouse model of Huntington's disease.

Neurobiology of disease

Chiodi V,Uchigashima M,Beggiato S,Ferrante A,Armida M,Martire A,Potenza RL,Ferraro L,Tanganelli S,Watanabe M,Domenici MR,Popoli P

PA1-745 was used in western blot to study the expression of CB1 receptors in GABAergic and glutamatergic neurons in a Huntington disease model

Thu Mar 01 00:00:00 UTC 2012

Overexpression of cannabinoid CB2 receptor in the brain induces hyperglycaemia and a lean phenotype in adult mice.

Journal of neuroendocrinology

Romero-Zerbo SY,Garcia-Gutierrez MS,Suárez J,Rivera P,Ruz-Maldonado I,Vida M,Rodriguez de Fonseca F,Manzanares J,Bermúdez-Silva FJ

PA1-745 was used in immunohistochemistry to study the effects of CB2 receptor overexpression in murine brain on body mass and glucose metabolism

Wed Aug 01 00:00:00 UTC 2012

Overexpression of cannabinoid CB2 receptor in the brain induces hyperglycaemia and a lean phenotype in adult mice.

Journal of neuroendocrinology

Romero-Zerbo SY,Garcia-Gutierrez MS,Suárez J,Rivera P,Ruz-Maldonado I,Vida M,Rodriguez de Fonseca F,Manzanares J,Bermúdez-Silva FJ

PA1-745 was used in immunohistochemistry to study the effects of CB2 receptor overexpression in murine brain on body mass and glucose metabolism

Wed Aug 01 00:00:00 UTC 2012

CB1 and CB2 cannabinoid receptor expression during development and in epileptogenic developmental pathologies.

Neuroscience

Zurolo E,Iyer AM,Spliet WG,Van Rijen PC,Troost D,Gorter JA,Aronica E

PA1-745 was used in immunohistochemistry to study the role of the CB1 and CB2 cannabinoid receptors in epileptogenic developmental pathologies

Wed Sep 29 00:00:00 UTC 2010

Anandamide suppresses proliferation and cytokine release from primary human T-lymphocytes mainly via CB2 receptors.

PloS one

Cencioni MT,Chiurchiù V,Catanzaro G,Borsellino G,Bernardi G,Battistini L,Maccarrone M

PA1-745 was used in flow cytometry to investigate the mechanism of human T cell regulation by anandamide

Thu Jan 14 00:00:00 UTC 2010

Adenosine A2A receptors enable the synaptic effects of cannabinoid CB1 receptors in the rodent striatum.

Journal of neurochemistry

Tebano MT,Martire A,Chiodi V,Pepponi R,Ferrante A,Domenici MR,Frank C,Chen JF,Ledent C,Popoli P

PA1-745 was used in western blot to study the role of adenosine A2A receptors in the synaptic effects of cannabinoid CB1 receptors in the rodent striatum

Tue Sep 01 00:00:00 UTC 2009

Cannabinoid-1 (CB1) receptors regulate colonic propulsion by acting at motor neurons within the ascending motor pathways in mouse colon.

American journal of physiology. Gastrointestinal and liver physiology

Sibaev A,Yüce B,Kemmer M,Van Nassauw L,Broedl U,Allescher HD,Göke B,Timmermans JP,Storr M

PA1-745 was used in immunohistochemistry to investigate the mechanism for the colonic propulsion mediated by cannabinoid-1 receptors

Thu Jan 01 00:00:00 UTC 2009

Ulcerative colitis induces changes on the expression of the endocannabinoid system in the human colonic tissue.

PloS one

Marquéz L,Suárez J,Iglesias M,Bermudez-Silva FJ,Rodríguez de Fonseca F,Andreu M

Published figure using Cannabinoid Receptor 1 polyclonal antibody (Product # PA1-745) in Immunohistochemistry

Fri Sep 04 00:00:00 UTC 2009

Effect of cannabidiol on sepsis-induced motility disturbances in mice: involvement of CB receptors and fatty acid amide hydrolase.

Neurogastroenterology and motility

de Filippis D,Iuvone T,d'amico A,Esposito G,Steardo L,Herman AG,Pelckmans PA,de Winter BY,de Man JG

PA1-745 was used in western blot to evaluate cannabidiol as a potential therapeutic for sepsis-induced ileus

Fri Aug 01 00:00:00 UTC 2008

FGF-2 promotes neurogenesis and neuroprotection and prolongs survival in a transgenic mouse model of Huntington's disease.

Proceedings of the National Academy of Sciences of the United States of America

Jin K,LaFevre-Bernt M,Sun Y,Chen S,Gafni J,Crippen D,Logvinova A,Ross CA,Greenberg DA,Ellerby LM

PA1-745 was used in western blot to evaluate the neuroprotective and neuroproliferative effects of FGF-2 in a transgenic mouse model of Huntingdon disease

Tue Dec 13 00:00:00 UTC 2005

Cloning of the first sn1-DAG lipases points to the spatial and temporal regulation of endocannabinoid signaling in the brain.

The Journal of cell biology

Bisogno T,Howell F,Williams G,Minassi A,Cascio MG,Ligresti A,Matias I,Schiano-Moriello A,Paul P,Williams EJ,Gangadharan U,Hobbs C,Di Marzo V,Doherty P

PA1-745 was used in immunohistochemistry to characterize sn1-DAG lipases.

Mon Nov 10 00:00:00 UTC 2003

Cloning of the first sn1-DAG lipases points to the spatial and temporal regulation of endocannabinoid signaling in the brain.

The Journal of cell biology

Bisogno T,Howell F,Williams G,Minassi A,Cascio MG,Ligresti A,Matias I,Schiano-Moriello A,Paul P,Williams EJ,Gangadharan U,Hobbs C,Di Marzo V,Doherty P

PA1-745 was used in immunohistochemistry to characterize sn1-DAG lipases.

Mon Nov 10 00:00:00 UTC 2003

Delta9-tetrahydrocannabinol selectively acts on CB1 receptors in specific regions of dorsal vagal complex to inhibit emesis in ferrets.

American journal of physiology. Gastrointestinal and liver physiology

Van Sickle MD,Oland LD,Mackie K,Davison JS,Sharkey KA

PA1-745 was used in immunohistochemistry to investigate the antiemetic effect of THC in the ferret

Mon Sep 01 00:00:00 UTC 2003

Bioinformatics

Protein Aliases: Brain-type cannabinoid receptor; CANN6; Cannabinoid receptor 1; cannabinoid receptor 1 (brain); CB-R; CB1; CB1 cannabinoid receptor; CB1 receptor; central cannabinoid receptor; HGNC:2159; OTTHUMP00000016839; RP1-23D17.1; seven transmembrane domains; G-protein-coupled receptor; striatal cannabinoid receptor type 1 protein

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Gene Aliases: CANN6; CB-R; CB1; CB1A; CB1K5; CB1R; CNR; CNR1; Skr6; SKR6R

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UniProt ID: (Human) P21554, (Mouse) P47746, (Rat) P20272

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Entrez Gene ID: (Human) 1268, (Chicken) 428633, (Mouse) 12801, (Rat) 25248

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Function(s)

cannabinoid receptor activity drug binding signal transducer activity G-protein coupled receptor activity protein binding G-protein coupled receptor

Process(es)

positive regulation of acute inflammatory response to antigenic stimulus G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger adenylate cyclase-modulating G-protein coupled receptor signaling pathway spermatogenesis axonal fasciculation aging response to nutrient memory positive regulation of neuron projection development sensory perception of pain positive regulation of fever generation negative regulation of fatty acid beta-oxidation regulation of synaptic transmission, GABAergic response to lipopolysaccharide negative regulation of mast cell activation negative regulation of dopamine secretion response to nicotine cannabinoid signaling pathway response to cocaine glucose homeostasis positive regulation of apoptotic process negative regulation of ion transport response to morphine response to ethanol negative regulation of action potential negative regulation of blood pressure positive regulation of blood pressure regulation of insulin secretion negative regulation of nitric-oxide synthase activity regulation of synaptic transmission, glutamatergic maternal process involved in female pregnancy regulation of feeding behavior regulation of penile erection signal transduction G-protein coupled receptor signaling pathway learning or memory regulation of lipid metabolic process

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Cannabinoid Receptor 1 Polyclonal Antibody

Cannabinoid Receptor 1 Antibody (PA1-745) in IHC

Product Details

PA1-745

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

in situ PLA (PLA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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