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Carbonic Anhydrase IX Antibody (PA5-77885) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of Carbonic Anhydrase IX was performed in 200 µM CoCl2 treated (right) or untreated (left) A431 cells fixed in ice cold MeOH for 5 min. Green: Carbonic Anhydrase IX Polyclonal Antibody (Product # PA5 77885) diluted at 1:500. Blue: Hoechst 33342 staining.
产品信息
PA5-77885
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein encompassing a sequence within the N-terminus region of human Carbonic Anhydrase IX. The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
0.97 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2735571
产品详细信息
Positive Control: HepG2 (untreated), HepG2 (200uM CoCl2 treatment for 24hr)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Carbonic anhydrase (CA) is an enzyme that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues. There are many genes that are inducible by hypoxia, via HIF-1 alpha. CA IX is one of the most inducible genes because of its stability and location within the membrane. Carbonic anhydrases have a widespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux. The pH is important in cell death under hypoxia, thus a blockade of CA IX results in increased cell death under hypoxia. Therefore, CA IX has become a reliable histochemical marker of hypoxia.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CA-9; CA-IX; Carbonate dehydratase IX; Carbonic anhydrase 9; Carbonic anhydrase IX; carbonic dehydratase; Membrane antigen MN; Membrane antigen MN homolog; P54 / 58N; P54/58N; pMW1; RCC-associated antigen G250; RCC-associated protein G250; Renal cell carcinoma-associated antigen G250
基因别名: CA9; CAIX; Car9; G250; MN; MN/CA9
UniProt ID: (Human) Q16790, (Mouse) Q8VHB5
Entrez Gene ID: (Human) 768, (Mouse) 230099
