Invitrogen

Carbonic Anhydrase IX Monoclonal Antibody (GT12)

Carbonic Anhydrase IX Antibody in Immunocytochemistry (ICC/IF)

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Carbonic Anhydrase IX Antibody (MA5-16318) in ICC/IF

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431 cells treated with 200 µM CoCl₂ for 48hr using anti-CAIX antibody [GT12] (Product # MA5-16318) at a dilution of 1:1,000.

Carbonic Anhydrase IX Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Carbonic Anhydrase IX Antibody in Western Blot (WB)

Carbonic Anhydrase IX Antibody in Flow Cytometry (Flow)

Carbonic Anhydrase IX Antibody in Immunohistochemistry (Frozen) (IHC (F))

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产品信息

MA5-16318

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:1,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫组化（冰冻） (IHC (F))

Assay-dependent

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免疫细胞化学 (ICC/IF)

1:100-1:1,000

流式细胞分析 (Flow)

1:100-1:1,000

免疫沉淀 (IP)

2-10 µg/mg of lysate

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

GT12

抗原

linear repetitive epitope in the PG region and allows for selective detection of both native and denatured Human CA IX.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2537837

产品详细信息

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Carbonic anhydrase (CA) is an enzyme that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues. There are many genes that are inducible by hypoxia, via HIF-1 alpha. CA IX is one of the most inducible genes because of its stability and location within the membrane. Carbonic anhydrases have a widespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux. The pH is important in cell death under hypoxia, thus a blockade of CA IX results in increased cell death under hypoxia. Therefore, CA IX has become a reliable histochemical marker of hypoxia.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Hypoxia-induced autophagy drives colorectal cancer initiation and progression by activating the PRKC/PKC-EZR (ezrin) pathway.

Autophagy

Qureshi-Baig K,Kuhn D,Viry E,Pozdeev VI,Schmitz M,Rodriguez F,Ullmann P,Koncina E,Nurmik M,Frasquilho S,Nazarov PV,Zuegel N,Boulmont M,Karapetyan Y,Antunes L,Val D,Mittelbronn M,Janji B,Haan S,Letellier E

MA5-16318 was used in Immunohistochemistry to observe significantly reduced in vivo tumor initiation and growth after a stable knockdown of ATG5.

Sat Aug 01 00:00:00 UTC 2020

Hypoxia-induced autophagy drives colorectal cancer initiation and progression by activating the PRKC/PKC-EZR (ezrin) pathway.

Autophagy

MA5-16318 was used in Immunohistochemistry to observe significantly reduced in vivo tumor initiation and growth after a stable knockdown of ATG5.

Sat Aug 01 00:00:00 UTC 2020

Linker stability influences the anti-tumor activity of acetazolamide-drug conjugates for the therapy of renal cell carcinoma.

Journal of controlled release : official journal of the Controlled Release Society

Cazzamalli S,Corso AD,Neri D

MA5-16318 was used in Immunohistochemistry-immunofluorescence to show that small molecule-drug conjugate (SMDC) products, obtained by the coupling of acetazolamide with monomethyl auristatin E (MMAE) using dipeptide linkers, display a potent anti-tumoral activity in mice bearing xenografted SKRC-52 renal cell carcinomas.

Sat Jan 28 00:00:00 UTC 2017

Mineralization of bone-related SaOS-2 cells under physiological hypoxic conditions.

The FEBS journal

Müller WE,Schröder HC,Tolba E,Diehl-Seifert B,Wang X

MA5-16318 was used in blocking or activating experiment and western blot to examine the metabolism of hypoxic SaOS-2 cells

Fri Jan 01 00:00:00 UTC 2016

生物信息学

蛋白别名: CA-9; CA-IX; Carbonate dehydratase IX; Carbonic anhydrase 9; Carbonic anhydrase IX; carbonic dehydratase; Membrane antigen MN; P54 / 58N; P54/58N; pMW1; RCC-associated antigen G250; RCC-associated protein G250; Renal cell carcinoma-associated antigen G250

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基因别名: CA9; CAIX; G250; MN

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UniProt ID: (Human) Q16790

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Entrez Gene ID: (Human) 768

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功能

carbonate dehydratase activity zinc ion binding

参与通路

morphogenesis of an epithelium one-carbon metabolic process bicarbonate transport response to testosterone response to drug secretion regulation of transcription from RNA polymerase II promoter in response to hypoxia