Invitrogen

Cardiac Myosin Heavy Chain Monoclonal Antibody (3-48)

Cardiac Myosin Heavy Chain Antibody in Western Blot (WB)

图: 1 / 1

Cardiac Myosin Heavy Chain Antibody (MA1-26180) in WB

Experimental and therapeutic medicine 2021 - Figure 2 HQQR improves Ang II-induced cardiomyocyte hypertrophy. (A) Immunofluorescence experiments were performed using anti-alpha-actinin antibodies to analyze cardiomyocyte hypertrophy (magnification, x200; scale bar, 50 um). (B) mRNA expression levels of the myocardial hypertrophy markers ANP, BNP and beta-MHC were examined using reverse transcription-quantitative PCR. (C) Protein expression levels of t... View More

Product Image Gallery

产品信息

MA1-26180

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

查看2篇已发表文章 2篇已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（冰冻） (IHC (F))

Assay-dependent

免疫细胞化学 (ICC/IF)

Assay-dependent

查看1篇已发表文章 1篇已发表文章

酶联免疫吸附实验 (ELISA)

Assay-dependent

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Bovine, Dog, Human, Mouse, Pig, Rabbit, Rat

已发表种属

Human, Mouse, Pig

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

3-48

抗原

Purified, full length, native human protein.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

IgG fraction

保存液

PBS, pH 7.4

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2147173

产品详细信息

This antibody is myosin alpha and beta heavy chain specific. Affinity constants: Human ventricular myosin heavy chains - 3.33 x 108M-1; Human atrial myosin heavy chains - 1.48 x 108M-1; Human skeletal muscle myosin - 1.06 x 108M-1; Canine cardiac myosin heavy chains - 2.06 x 108M-1. This antibody cross-reacts with heavy chains of human myosin isolated from slow skeletal muscle, fast skeletal muscle, eye muscle, and streptococcal M5 peptide. It does not react with human platelets, smooth muscle myosin heavy chains, myosin light chains, myosin fragments circulating in blood of patients with myocardial infarction, nor does it react with any other human tissue antigens or blood cells.

Recommended positive control: Slow skeletal muscle, fast skeletal muscle, eye muscle.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Insertion of an immunodominant T helper cell epitope within the Group A Streptococcus M protein promotes an IFN-γ-dependent shift from a non-protective to a protective immune response.

Frontiers in immunology

Emami S,Rojas Converso T,Persson JJ,Johansson-Lindbom B

MA1-26180 was used in ELISA to suggest that inclusion of the 2W T cell epitope in the M protein confers essential qualitative alterations in the adaptive immune response against GAS, and that sparsity in IFN-γ-promoting Th cell epitopes in the M protein may constitute an immune evasion mechanism, evolved to allow the pathogen to avoid attack by complement-fixing antibodies.

Mon Sep 04 00:00:00 UTC 2023

Circulating hemopexin modulates anthracycline cardiac toxicity in patients and in mice.

Science advances

Liu J,Lane S,Lall R,Russo M,Farrell L,Debreli Coskun M,Curtin C,Araujo-Gutierrez R,Scherrer-Crosbie M,Trachtenberg BH,Kim J,Tolosano E,Ghigo A,Gerszten RE,Asnani A

MA1-26180 was used in Immunohistochemistry to investigate if heme-binding protein hemopexin induction represents a compensatory response during doxorubicin treatment.

Fri Dec 23 00:00:00 UTC 2022

Huoxue Qianyang Qutan recipe attenuates Ang II-induced cardiomyocyte hypertrophy by regulating reactive oxygen species production.

Experimental and therapeutic medicine

Gui M,Yao L,Lu B,Wang J,Zhou X,Li J,Dong Z,Fu D

Published figure using Cardiac Myosin Heavy Chain monoclonal antibody (Product # MA1-26180) in Western Blot

Wed Dec 01 00:00:00 UTC 2021

Intercellular cGAMP transmission induces innate immune activation and tissue inflammation in Trex1 deficiency.

iScience

Jütte BB,Krollmann C,Cieslak K,Koerber RM,Boor P,Graef CM,Bartok E,Wagner M,Carell T,Landsberg J,Aymans P,Wenzel J,Brossart P,Teichmann LL

MA1-26180 was used in ELISA to demonstrate that, in Trex1 -/- -associated autoimmunity, cGAMP shuttling from radioresistant to immune cells induces NF-κB activation, interferon regulatory factor 3 (IRF3) phosphorylation, and subsequent interferon signaling. cGAMP travel prevented myeloid cell and lymphocyte death, promoting their accumulation in secondary lymphoid tissue.

Fri Aug 20 00:00:00 UTC 2021

Cardiosphere-derived exosomal microRNAs for myocardial repair in pediatric dilated cardiomyopathy.

Science translational medicine

Hirai K,Ousaka D,Fukushima Y,Kondo M,Eitoku T,Shigemitsu Y,Hara M,Baba K,Iwasaki T,Kasahara S,Ohtsuki S,Oh H

MA1-26180 was used in Immunohistochemistry to determine the safety and efficacy of cardiosphere-derived cells in a porcine model of dilated cardiomyopathy and translate the preclinical results into this patient population.

Wed Dec 09 00:00:00 UTC 2020

Cardiosphere-derived exosomal microRNAs for myocardial repair in pediatric dilated cardiomyopathy.

Science translational medicine

Hirai K,Ousaka D,Fukushima Y,Kondo M,Eitoku T,Shigemitsu Y,Hara M,Baba K,Iwasaki T,Kasahara S,Ohtsuki S,Oh H

Wed Dec 09 00:00:00 UTC 2020

Defining the molecular signatures of human right heart failure.

Life sciences

Williams JL,Cavus O,Loccoh EC,Adelman S,Daugherty JC,Smith SA,Canan B,Janssen PML,Koenig S,Kline CF,Mohler PJ,Bradley EA

MA1-26180 was used in Western Blotting to provide some of the first analyses of the molecular heterogeneity between human RV and LV tissue, as well as key differences in human disease (RVF secondary to pulmonary hypertension and LVAD mediated RVF).

Thu Mar 01 00:00:00 UTC 2018

Generation of multipotent induced cardiac progenitor cells from mouse fibroblasts and potency testing in ex vivo mouse embryos.

Nature protocols

Lalit PA,Rodriguez AM,Downs KM,Kamp TJ

MA126180 was used in immunocytochemistry to develop a protocol to generate expandable and multipotent induced cardiac progenitor cells from mouse adult fibroblasts

Mon May 01 00:00:00 UTC 2017

生物信息学

蛋白别名: alpha cardiac MHC; alpha myosin; alpha-cardiac myosin heavy chain; Antigen MLAA-21; beta cardiac myosin heavy chain; beta myosin heavy chain; beta-myosin heavy chain; cardiac muscle myosin heavy chain 7 beta; cardiac myosin heavy chain alpha isoform; cardiac myosin heavy chain beta isoform; cardiomyopathy, hypertrophic 1; MYH-beta/slow; MyHC-alpha; MyHC-beta; MyHC-slow; myopathy, distal 1; myosin 7; Myosin cardiac muscle beta chain; Myosin heavy chain 6; Myosin heavy chain 7; Myosin heavy chain 7B, cardiac muscle beta isoform; myosin heavy chain polypeptide 6 cardiac muscle adult; myosin heavy chain polypeptide 7 cardiac muscle fetal; Myosin heavy chain slow isoform; myosin heavy chain slow type 1 (beta cardiac); Myosin heavy chain, cardiac muscle alpha isoform; Myosin heavy chain, cardiac muscle alpha isoform (MyHC-alpha); Myosin heavy chain, cardiac muscle beta isoform; myosin heavy chain, cardiac muscle, adult; myosin heavy chain, cardiac muscle, fetal; myosin heavy chain, polypeptide 6, cardiac muscle, alpha; myosin heavy chain, polypeptide 7; myosin, heavy chain 6, cardiac muscle, alpha; myosin, heavy chain 6, cardiac muscle, alpha (cardiomyopathy, hypertrophic 1); myosin, heavy chain 7, cardiac muscle, beta; myosin, heavy chain 7B, cardiac muscle, beta; myosin, heavy polypeptide 6, cardiac muscle, alpha; myosin, heavy polypeptide 6, cardiac muscle, alpha (cardiomyopathy, hypertrophic 1); myosin, heavy polypeptide 7, cardiac muscle, beta; myosin, heavy polypeptide 7B, cardiac muscle, beta; Myosin-6; Myosin-7; Myosin-7B; rhabdomyosarcoma antigen MU-RMS-40.7A; Slow A MYH14; type slow/beta myosin heavy chain; U937-associated antigen

查看更多收起

基因别名: A830009F23Rik; AA517445; alpha-MHC; ASD3; B-MHC; beta-MHC; BOS_10147; CMD1EE; CMD1S; CMH1; CMH14; KIAA1512; MHC14; MPD1; MYH14; MYH6; MYH7; MYH7B; MYHC; Myhc-a; Myhc-b; MyHC-I; MYHCA; MYHCB; SPMD; SPMM; SSS3