Invitrogen

Cardiac Myosin Heavy Chain Monoclonal Antibody (3-48)

Cardiac Myosin Heavy Chain Antibody in Western Blot (WB)

FIGURE: 1 / 1

Cardiac Myosin Heavy Chain Antibody (MA1-26180) in WB

Experimental and therapeutic medicine 2021 - Figure 2 HQQR improves Ang II-induced cardiomyocyte hypertrophy. (A) Immunofluorescence experiments were performed using anti-alpha-actinin antibodies to analyze cardiomyocyte hypertrophy (magnification, x200; scale bar, 50 um). (B) mRNA expression levels of the myocardial hypertrophy markers ANP, BNP and beta-MHC were examined using reverse transcription-quantitative PCR. (C) Protein expression levels of t... View More

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Product Details

MA1-26180

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Bovine, Dog, Human, Mouse, Pig, Rabbit, Rat

Published species

Human, Mouse, Pig

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3-48

Immunogen

Purified, full length, native human protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

IgG fraction

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2147173

Product Specific Information

This antibody is myosin alpha and beta heavy chain specific. Affinity constants: Human ventricular myosin heavy chains - 3.33 x 108M-1; Human atrial myosin heavy chains - 1.48 x 108M-1; Human skeletal muscle myosin - 1.06 x 108M-1; Canine cardiac myosin heavy chains - 2.06 x 108M-1. This antibody cross-reacts with heavy chains of human myosin isolated from slow skeletal muscle, fast skeletal muscle, eye muscle, and streptococcal M5 peptide. It does not react with human platelets, smooth muscle myosin heavy chains, myosin light chains, myosin fragments circulating in blood of patients with myocardial infarction, nor does it react with any other human tissue antigens or blood cells.

Recommended positive control: Slow skeletal muscle, fast skeletal muscle, eye muscle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Insertion of an immunodominant T helper cell epitope within the Group A Streptococcus M protein promotes an IFN-γ-dependent shift from a non-protective to a protective immune response.

Frontiers in immunology

Emami S,Rojas Converso T,Persson JJ,Johansson-Lindbom B

MA1-26180 was used in ELISA to suggest that inclusion of the 2W T cell epitope in the M protein confers essential qualitative alterations in the adaptive immune response against GAS, and that sparsity in IFN-γ-promoting Th cell epitopes in the M protein may constitute an immune evasion mechanism, evolved to allow the pathogen to avoid attack by complement-fixing antibodies.

Mon Sep 04 00:00:00 UTC 2023

Circulating hemopexin modulates anthracycline cardiac toxicity in patients and in mice.

Science advances

Liu J,Lane S,Lall R,Russo M,Farrell L,Debreli Coskun M,Curtin C,Araujo-Gutierrez R,Scherrer-Crosbie M,Trachtenberg BH,Kim J,Tolosano E,Ghigo A,Gerszten RE,Asnani A

MA1-26180 was used in Immunohistochemistry to investigate if heme-binding protein hemopexin induction represents a compensatory response during doxorubicin treatment.

Fri Dec 23 00:00:00 UTC 2022

Huoxue Qianyang Qutan recipe attenuates Ang II-induced cardiomyocyte hypertrophy by regulating reactive oxygen species production.

Experimental and therapeutic medicine

Gui M,Yao L,Lu B,Wang J,Zhou X,Li J,Dong Z,Fu D

Published figure using Cardiac Myosin Heavy Chain monoclonal antibody (Product # MA1-26180) in Western Blot

Wed Dec 01 00:00:00 UTC 2021

Intercellular cGAMP transmission induces innate immune activation and tissue inflammation in Trex1 deficiency.

iScience

Jütte BB,Krollmann C,Cieslak K,Koerber RM,Boor P,Graef CM,Bartok E,Wagner M,Carell T,Landsberg J,Aymans P,Wenzel J,Brossart P,Teichmann LL

MA1-26180 was used in ELISA to demonstrate that, in Trex1 -/- -associated autoimmunity, cGAMP shuttling from radioresistant to immune cells induces NF-κB activation, interferon regulatory factor 3 (IRF3) phosphorylation, and subsequent interferon signaling. cGAMP travel prevented myeloid cell and lymphocyte death, promoting their accumulation in secondary lymphoid tissue.

Fri Aug 20 00:00:00 UTC 2021

Cardiosphere-derived exosomal microRNAs for myocardial repair in pediatric dilated cardiomyopathy.

Science translational medicine

Hirai K,Ousaka D,Fukushima Y,Kondo M,Eitoku T,Shigemitsu Y,Hara M,Baba K,Iwasaki T,Kasahara S,Ohtsuki S,Oh H

MA1-26180 was used in Immunohistochemistry to determine the safety and efficacy of cardiosphere-derived cells in a porcine model of dilated cardiomyopathy and translate the preclinical results into this patient population.

Wed Dec 09 00:00:00 UTC 2020

Cardiosphere-derived exosomal microRNAs for myocardial repair in pediatric dilated cardiomyopathy.

Science translational medicine

Hirai K,Ousaka D,Fukushima Y,Kondo M,Eitoku T,Shigemitsu Y,Hara M,Baba K,Iwasaki T,Kasahara S,Ohtsuki S,Oh H

Wed Dec 09 00:00:00 UTC 2020

Defining the molecular signatures of human right heart failure.

Life sciences

Williams JL,Cavus O,Loccoh EC,Adelman S,Daugherty JC,Smith SA,Canan B,Janssen PML,Koenig S,Kline CF,Mohler PJ,Bradley EA

MA1-26180 was used in Western Blotting to provide some of the first analyses of the molecular heterogeneity between human RV and LV tissue, as well as key differences in human disease (RVF secondary to pulmonary hypertension and LVAD mediated RVF).

Thu Mar 01 00:00:00 UTC 2018

Generation of multipotent induced cardiac progenitor cells from mouse fibroblasts and potency testing in ex vivo mouse embryos.

Nature protocols

Lalit PA,Rodriguez AM,Downs KM,Kamp TJ

MA126180 was used in immunocytochemistry to develop a protocol to generate expandable and multipotent induced cardiac progenitor cells from mouse adult fibroblasts

Mon May 01 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: alpha cardiac MHC; alpha myosin; alpha-cardiac myosin heavy chain; Antigen MLAA-21; beta cardiac myosin heavy chain; beta myosin heavy chain; beta-myosin heavy chain; cardiac muscle myosin heavy chain 7 beta; cardiac myosin heavy chain alpha isoform; cardiac myosin heavy chain beta isoform; cardiomyopathy, hypertrophic 1; MYH-beta/slow; MyHC-alpha; MyHC-beta; MyHC-slow; myopathy, distal 1; myosin 7; Myosin cardiac muscle beta chain; Myosin heavy chain 6; Myosin heavy chain 7; Myosin heavy chain 7B, cardiac muscle beta isoform; myosin heavy chain polypeptide 6 cardiac muscle adult; myosin heavy chain polypeptide 7 cardiac muscle fetal; Myosin heavy chain slow isoform; myosin heavy chain slow type 1 (beta cardiac); Myosin heavy chain, cardiac muscle alpha isoform; Myosin heavy chain, cardiac muscle alpha isoform (MyHC-alpha); Myosin heavy chain, cardiac muscle beta isoform; myosin heavy chain, cardiac muscle, adult; myosin heavy chain, cardiac muscle, fetal; myosin heavy chain, polypeptide 6, cardiac muscle, alpha; myosin heavy chain, polypeptide 7; myosin, heavy chain 6, cardiac muscle, alpha; myosin, heavy chain 6, cardiac muscle, alpha (cardiomyopathy, hypertrophic 1); myosin, heavy chain 7, cardiac muscle, beta; myosin, heavy chain 7B, cardiac muscle, beta; myosin, heavy polypeptide 6, cardiac muscle, alpha; myosin, heavy polypeptide 6, cardiac muscle, alpha (cardiomyopathy, hypertrophic 1); myosin, heavy polypeptide 7, cardiac muscle, beta; myosin, heavy polypeptide 7B, cardiac muscle, beta; Myosin-6; Myosin-7; Myosin-7B; rhabdomyosarcoma antigen MU-RMS-40.7A; Slow A MYH14; type slow/beta myosin heavy chain; U937-associated antigen

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Gene Aliases: A830009F23Rik; AA517445; alpha-MHC; ASD3; B-MHC; beta-MHC; BOS_10147; CMD1EE; CMD1S; CMH1; CMH14; KIAA1512; MHC14; MPD1; MYH14; MYH6; MYH7; MYH7B; MYHC; Myhc-a; Myhc-b; MyHC-I; MYHCA; MYHCB; SPMD; SPMM; SSS3

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UniProt ID: (Human) P13533, (Bovine) Q9BE39, (Human) P12883, (Pig) P79293, (Dog) P49824, (Human) A7E2Y1, (Mouse) Q02566, (Rat) P02564, (Mouse) Q3TZN2, (Mouse) A2AQP0

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Entrez Gene ID: (Human) 4624, (Dog) 100685644, (Rabbit) 100125987, (Rabbit) 100101566, (Bovine) 282714, (Human) 4625, (Pig) 396860, (Dog) 403807, (Human) 57644, (Dog) 477206, (Mouse) 17888, (Rat) 29556, (Rat) 29557, (Mouse) 140781, (Rat) 311570, (Mouse) 668940

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Function(s)

microfilament motor activity actin binding calmodulin binding ATP binding ATPase activity myosin phosphatase activity protein kinase binding actin-dependent ATPase activity motor activity protein binding protein homodimerization activity protein heterodimerization activity nucleotide binding calcium-dependent ATPase activity identical protein binding

Process(es)

in utero embryonic development regulation of the force of heart contraction regulation of heart rate muscle contraction striated muscle contraction adult heart development visceral muscle development regulation of heart contraction metabolic process regulation of blood pressure actin filament-based movement muscle filament sliding myofibril assembly BMP signaling pathway regulation of ATPase activity sarcomere organization ATP metabolic process cardiac muscle fiber development atrial cardiac muscle tissue morphogenesis ventricular cardiac muscle tissue morphogenesis cardiac muscle contraction canonical Wnt signaling pathway regulation of heart growth response to heat response to reactive oxygen species transition between fast and slow fiber skeletal muscle contraction regulation of the force of skeletal muscle contraction regulation of slow-twitch skeletal muscle fiber contraction protein dephosphorylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cardiac Myosin Heavy Chain Monoclonal Antibody (3-48)

Cardiac Myosin Heavy Chain Antibody (MA1-26180) in WB

Product Details

MA1-26180

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Bioinformatics

Performance Guarantee

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