Invitrogen

Caspase 1 Polyclonal Antibody

Caspase 1 Antibody in Immunocytochemistry (ICC/IF)

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Caspase 1 Antibody (PA5-17570) in ICC/IF

Immunofluorescence analysis of Caspase 1 was performed using 70% confluent log phase THP-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Caspase 1 Polyclonal Antibody (Product # PA5-17570) at 1:100 dilution in 0.1% BSA, incubated at 4 degree cel... View More

Caspase 1 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

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产品信息

PA5-17570

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看3篇已发表文章 3篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:100

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues within the p20 subunit of human caspase-1

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

125 µg/mL

纯化类型

Antigen affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10987516

产品详细信息

PA5-17570 detects Caspase 1 in WB application with human samples.

It is not recommended to aliquot this antibody.

靶标信息

Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1-b and IL-18. Caspase-1 was initially identified as an IL-1-b-converting enzyme; later experiments revealed it to be a mammalian homolog of the C. elegans cell death gene ced-3 whose overexpression can induce apoptosis in fibroblasts.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Excess fatty acids induce pancreatic acinar cell pyroptosis through macrophage M1 polarization.

BMC gastroenterology

Xia W,Lu Z,Chen W,Zhou J,Zhao Y

PA5-17570 was used in Immunohistochemistry (PFA fixed), Western Blot to conclude that cathepsin S may be a new target for the clinical treatment of hyperlipidemic pancreatitis.

Sat Feb 19 00:00:00 UTC 2022

Excess fatty acids induce pancreatic acinar cell pyroptosis through macrophage M1 polarization.

BMC gastroenterology

Xia W,Lu Z,Chen W,Zhou J,Zhao Y

PA5-17570 was used in Immunohistochemistry (PFA fixed), Western Blot to conclude that cathepsin S may be a new target for the clinical treatment of hyperlipidemic pancreatitis.

Sat Feb 19 00:00:00 UTC 2022

Excess fatty acids induce pancreatic acinar cell pyroptosis through macrophage M1 polarization.

BMC gastroenterology

Xia W,Lu Z,Chen W,Zhou J,Zhao Y

PA5-17570 was used in Immunohistochemistry (PFA fixed), Western Blot to conclude that cathepsin S may be a new target for the clinical treatment of hyperlipidemic pancreatitis.

Sat Feb 19 00:00:00 UTC 2022

Exenatide Attenuates Non-Alcoholic Steatohepatitis by Inhibiting the Pyroptosis Signaling Pathway.

Frontiers in endocrinology

Liu Y,Wang DW,Wang D,Duan BH,Kuang HY

Published figure using Caspase 1 polyclonal antibody (Product # PA5-17570) in Immunocytochemistry

Mon Dec 20 00:00:00 UTC 2021

Burkholderia pseudomallei triggers canonical inflammasome activation in a human primary macrophage-based infection model.

PLoS neglected tropical diseases

Lichtenegger S,Stiehler J,Saiger S,Zauner A,Kleinhappl B,Bernecker C,Schlenke P,Wagner GE,Krause K,Gastager M,Steinmetz I

PA5-17570 was used in Western Blotting to develop a an in vitro B. pseudomallei infection model and study the process of B. pseudomallei-triggered cell death and bacterial elimination in those models.

Sun Nov 01 00:00:00 UTC 2020

Burkholderia pseudomallei triggers canonical inflammasome activation in a human primary macrophage-based infection model.

PLoS neglected tropical diseases

Lichtenegger S,Stiehler J,Saiger S,Zauner A,Kleinhappl B,Bernecker C,Schlenke P,Wagner GE,Krause K,Gastager M,Steinmetz I

Sun Nov 01 00:00:00 UTC 2020

Priming and Activation of Inflammasome by Canarypox Virus Vector ALVAC via the cGAS/IFI16-STING-Type I IFN Pathway and AIM2 Sensor.

Journal of immunology (Baltimore, Md. : 1950)

Liu F,Niu Q,Fan X,Liu C,Zhang J,Wei Z,Hou W,Kanneganti TD,Robb ML,Kim JH,Michael NL,Sun J,Soong L,Hu H

PA5-17570 was used to measure the caspase-1 protein in ALVAC infected THP-1 cells.

Wed Nov 01 00:00:00 UTC 2017

生物信息学

蛋白别名: CASP-1; CASP1 nirs variant 1; caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase); Caspase-1; Caspase1; ICE; IL-1 beta-converting enzyme; IL-1BC; IL1B-convertase; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45

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基因别名: CASP1; ICE; IL1BC; IL1BCE; P45

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UniProt ID: (Human) P29466

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Entrez Gene ID: (Human) 834

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功能

endopeptidase activity cysteine-type endopeptidase activity protein binding cysteine-type endopeptidase activator activity involved in apoptotic process cysteine-type endopeptidase activity involved in apoptotic process protease

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