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Caspase 1 (cleaved Asp297) Antibody (PA5-77886) in WB
Knockout of Caspase 1 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR917229_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of Caspase 1 was performed by loading 30 µg of HT29 Cas9 (Lane 1), HT29 Cas9 treated with 0.1 µg/mL of LPS for 4hrs followed by 1mM of ATP for 2hrs (Lane 2), &(8)203;HT29 CASP1 KO (Lane 3) and... View More
产品信息
PA5-77886
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Human Caspase 1 (cleaved Asp297). The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2735573
产品详细信息
Positive Control: conditioned medium of treated THP-1, THP-1 conditioned medium (500 ng/mL LPS treatment for 4 hr in serum free and 1 mM ATP treatment for 1.5 hr)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Caspase 1 (cleaved Asp297) encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. Caspase 1 (cleaved Asp297) was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. Caspase 1 (cleaved Asp297) has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CASP-1; CASP1 nirs variant 1; caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase); Caspase-1; ICE; IL-1 beta-converting enzyme; IL-1BC; IL1B-convertase; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45
基因别名: CASP1; ICE; IL1BC; IL1BCE; P45
UniProt ID: (Human) P29466
Entrez Gene ID: (Human) 834
response to hypoxia
proteolysis
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
signal transduction
regulation of autophagy
protein processing
response to lipopolysaccharide
interleukin-1 beta production
response to ATP
regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of interleukin-1 alpha secretion
positive regulation of interleukin-1 beta secretion
regulation of inflammatory response
mitochondrial depolarization
membrane hyperpolarization
pyroptosis
cellular response to mechanical stimulus
cellular response to organic substance
programmed necrotic cell death
toxin transport
