Invitrogen

Caspase 12 Polyclonal Antibody

3个已发表图片

2 篇参考文献

查看其他9个Caspase 12抗体

抗体检测数据 (3)
已发表图片 (3)

Caspase 12 Antibody in Immunocytochemistry (ICC/IF)

Caspase 12 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 6

Caspase 12 Antibody (PA5-19963) in ICC/IF

Immunofluorescence of Caspase-12 in Mouse Liver cells with Caspase 12 Polyclonal Antibody (Product # PA5-19963) at 10 µg/mL.

Caspase 12 Antibody in Immunocytochemistry (ICC/IF)

Caspase 12 Antibody in Immunohistochemistry (IHC)

Caspase 12 Antibody in Western Blot (WB)

Caspase 12 Antibody in Western Blot (WB)

Caspase 12 Antibody in Western Blot (WB)

Caspase 12 Antibody in Western Blot (WB)

Product Image Gallery

Caspase 12 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence of Caspase-12 in Mouse Liver cells with Caspase 12 Polyclonal Antibody (Product # PA5-19963) at 10 µg/mL.

Caspase 12 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of mouse liver tissue using Caspase 12 Polyclonal Antibody (Product # PA5-19963) at 2 µg/mL.

Caspase 12 Antibody in Western Blot (WB) — Western Blot analysis of Caspase-12 in (A) human spleen and (B) mouse spleen tissue lysate with Caspase 12 Polyclonal Antibody (Product # PA5-19963) at 1 µg/mL.

Caspase 12 Antibody in Western Blot (WB) — FIGURE 5 Inhibition of autophagy abolishes PNS protection on TG-induced ER stress response and associated apoptosis. (A) SiNC or siATG7 transfected H9c2 cells, either untreated (CN group) or pretreated with 40 mug/ml PNS for 12 h before addition of 1 muM TG (TG group or PNS plus TG group) for 12 h were immunofluorescenced with the primary anti-calnexin antibody and imaged by a laser scanning confocal microscopy. Scale bar: 30 mum; in box: 10 mum. (B) SiNC or siATG7 transfected H9c2 cells, treated as in A, were immunoblotted with the antibodies to BiP, CHOP, Cleaved Caspase-3 and Caspase-12 as well as beta -actin. Bands were quantified relative to beta -actin by densitometry (Mean +- SEM; ** p < 0.01 relative to CN group, or indicated group). (C) SiNC or siATG7 transfected H9c2 cells, treated as in A, were stained with TUNEL and imaged by a laser scanning confocal microscopy. Scale bar: 200 mum. Bar graph shows the percentage of TUNEL positive cells (Mean +- SEM; 200-250 cells; ** p < 0.01 relative to CN group, or indicated group).

Caspase 12 Antibody in Western Blot (WB) — Figure 2. DZX regulates the expression of CHOP, GRP78, caspase-12 and caspase-3 in OGD-treated hippocampal cells. (A) Immunohistochemical staining demonstrated the distribution of GRP78 and CHOP in hippocampal cells exposed to OGD. (B) Western blot analysis was used to detect the expression of GRP78 and CHOP in hippocampal cells exposed to OGD. (C) Protein expression levels of caspase-12 and caspase-3 in hippocampal cells exposed to OGD were detected using western blotting. Data are expressed as the mean +- standard deviation. *P<0.05, as indicated. DZX, diazoxide; CHOP, C/EBP homologous protein; GRP78, 78 kDa glucose-regulated protein; OGD, oxygen-glucose deprivation; 5-HD, 5-hydroxydecanoate.

Caspase 12 Antibody in Western Blot (WB) — Figure 5. Pretreatment with DZX modulates the protein expression of Bcl-2, caspase-12, caspase-3, Bax, CHOP and GRP78 in rats subjected to I/R. Western blot analysis was used to detect the protein expression levels of Bcl-2, caspase-12, caspase-3, Bax, CHOP and GRP78 in hippocampal cells isolated from I/R-treated rats. Rats were treated with DZX with or without 5-HD and subjected to cerebral ischemia for 2 h followed by 12 h of reperfusion. Sham rats were not subjected to cerebral ischemia. DZX, diazoxide; Bcl-2, B-cell lymphoma-2; Bax, Bcl-2-associated X protein; CHOP, C/EBP homologous protein; GRP78, 78 kDa glucose-regulated protein; I/R, ischemia/reperfusion; 5-HD, 5-hydroxydecanoate.

产品信息

PA5-19963

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

查看2篇已发表文章 2篇已发表文章

免疫组化 (IHC)

2 µg/mL

-

免疫细胞化学 (ICC/IF)

10 µg/mL

-

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A peptide corresponding to amino acids 90 to 140 of murine caspase-12.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_11153731

产品详细信息

A suggested positive control is mouse brain tissue lysate.

PA5-19963 can be used with blocking peptide PEP-0088.

靶标信息

Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the mains, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including -gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Inhibition of Autophagy Prevents Panax Notoginseng Saponins (PNS) Protection on Cardiac Myocytes Against Endoplasmic Reticulum (ER) Stress-Induced Mitochondrial Injury, Ca²⁺ Homeostasis and Associated Apoptosis.

Frontiers in pharmacology

Chen J,Li L,Bai X,Xiao L,Shangguan J,Zhang W,Zhang X,Wang S,Liu G

PA5-19963 was used in Western Blotting to investigate the roles of PNS played in TG-induced mitochondrial function, ROS accumulation and autophagy.

Fri Mar 26 00:00:00 UTC 2021

Inhibition of Autophagy Prevents Panax Notoginseng Saponins (PNS) Protection on Cardiac Myocytes Against Endoplasmic Reticulum (ER) Stress-Induced Mitochondrial Injury, Ca²⁺ Homeostasis and Associated Apoptosis.

Frontiers in pharmacology

Chen J,Li L,Bai X,Xiao L,Shangguan J,Zhang W,Zhang X,Wang S,Liu G

PA5-19963 was used in Western Blotting to investigate the roles of PNS played in TG-induced mitochondrial function, ROS accumulation and autophagy.

Fri Mar 26 00:00:00 UTC 2021

Diazoxide inhibits of ER stress‑mediated apoptosis during oxygen‑glucose deprivation in vitro and cerebral ischemia‑reperfusion in vivo.

Molecular medicine reports

Lei X,Lei L,Zhang Z,Cheng Y

Published figure using Caspase 12 polyclonal antibody (Product # PA5-19963) in Western Blot

Fri Jun 01 00:00:00 UTC 2018

生物信息学

蛋白别名: CASP-12; caspase 12 pseudogene 1; Caspase-12; Inactive caspase-12; OTTMUSP00000045799; OTTMUSP00000045802; OTTMUSP00000045803; UNQ9415

查看更多收起

基因别名: CASP-12; CASP12; CASP12P1; UNQ9415/PRO34398

查看更多收起

UniProt ID: (Human) Q6UXS9, (Rat) Q920D5, (Mouse) O08736

查看更多收起

Entrez Gene ID: (Human) 100506742, (Rat) 156117, (Mouse) 12364

查看更多收起

cysteine-type endopeptidase activity involved in apoptotic process protease binding endopeptidase activity cysteine-type endopeptidase activity protein binding cysteine-type peptidase activity peptidase activity hydrolase activity

endoplasmic reticulum unfolded protein response regulation of apoptotic process regulation of inflammatory response intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress self proteolysis proteolysis apoptotic process aging response to cold response to inorganic substance response to iron(III) ion response to organonitrogen compound protein autoprocessing response to anoxia response to endoplasmic reticulum stress response to nicotine response to drug positive regulation of apoptotic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process response to ethanol execution phase of apoptosis intrinsic apoptotic signaling pathway