Invitrogen

Caspase 12 Polyclonal Antibody

Caspase 12 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Caspase 12 Antibody (PA5-87375) in IHC (P)

Immunohistochemistry analysis of Caspase 12 in paraffin-embedded mouse spleen. Samples were incubated with Caspase 12 Polyclonal antibody (Product # PA5-87375) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Caspase 12 Antibody in Western Blot (WB)

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产品信息

PA5-87375

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Caspase-12 (NP_0011779452)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.368 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2804102

产品详细信息

Immunogen sequence: FNNRNCQSLK DKPKVIIMQA CRGNGAGIVW FTTDSGKASA DTHGRLLQGN ICNDAVTKAH VEKDFIAFKS STPHNVSWRH ETNGSVFISQ IIYYFREYSW S

Positive Samples: Jurkat, Mouse lung, Mouse ovary, Mouse uterus, Rat lung

靶标信息

Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the mains, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including -gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues.

仅用于科研。不用于诊断过程。未经明确授权不得转售。