Invitrogen

Caspase 9 Polyclonal Antibody

Caspase 9 Antibody in Immunocytochemistry (ICC/IF)

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Caspase 9 Antibody (PA5-22252) in ICC/IF

Immunofluorescence analysis of Caspase 9 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Caspase 9 Rabbit Polyclonal Antibody(Product # PA5-22252) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius o... View More

Caspase 9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-22252

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

5 µg/mL

免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Human, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 88 and 416 of Caspase 9 (Uniprot ID#P55211)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.55 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_11152725

产品详细信息

Recommended positive controls: HeLa (30 µM cisplatin treatment for 24 hr), NTUB1.

Predicted reactivity: Dog (80%), Bovine (82%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Caspase 9 (ICE-like apoptotic protease 6, ICE LAP6, apoptotic protease Mch6, apoptotic protease activating factor 3, Apaf3) is a member of the peptidase family C14 that contains a CARD domain. Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspase 9 is active as a heterotetramer, is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Further, Caspase 9 is involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf- 1 leads to activation of the protease which then cleaves and activates caspase-3. Caspase 9 promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner, and proteolytically cleaves poly(ADP- ribose) polymerase (PARP). Caspase 9 is expressed in variety of human tissues. An isoform of rat Caspase-9 has been identified in which the C terminus of full-length Caspase-9 is replaced with an alternative peptide sequence. Mutations in Caspase 9 affect brain development. In mice, insufficient Caspase 9 have resulted in abnormal cerebrum development and neuron development. In humans, dysfunctional Caspase 9 expression vary from tissue to tissue. Low levels of Caspase 9 may play a role in cancer development and neurodegenerative diseases such as Alzheimer’s Disease (AD).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Anti-Apoptotic Effect of Apelin in Human Placenta: Studies on BeWo Cells and Villous Explants from Third-Trimester Human Pregnancy.

International journal of molecular sciences

Mlyczyńska E,Myszka M,Kurowska P,Dawid M,Milewicz T,Bałajewicz-Nowak M,Kowalczyk P,Rak A

Published figure using Caspase 9 polyclonal antibody (Product # PA5-22252) in Western Blot

Tue Mar 09 00:00:00 UTC 2021

E2F5 Promotes the Malignancy of Ovarian Cancer Via the Regulation of Hippo and Wnt Pathways.

Genetic testing and molecular biomarkers

Malgundkar SH,Burney I,Al Moundhri M,Al Kalbani M,Lakhtakia R,Okamoto A,Tamimi Y

PA5-22252 was used in Western Blot to conclude that E2F5 promotes ovarian carcinogenesis via the regulation of Hippo and Wnt pathways.

Mon Mar 01 00:00:00 UTC 2021

FAT4 silencing promotes epithelial-to-mesenchymal transition and invasion via regulation of YAP and β-catenin activity in ovarian cancer.

BMC cancer

Malgundkar SH,Burney I,Al Moundhri M,Al Kalbani M,Lakhtakia R,Okamoto A,Tamimi Y

PA5-22252 was used in Western Blotting to examine the role of FAT4 in proliferation and metastasis, and its mechanisms of interaction in these processes.

Mon May 04 00:00:00 UTC 2020

FAT4 silencing promotes epithelial-to-mesenchymal transition and invasion via regulation of YAP and β-catenin activity in ovarian cancer.

BMC cancer

Malgundkar SH,Burney I,Al Moundhri M,Al Kalbani M,Lakhtakia R,Okamoto A,Tamimi Y

PA5-22252 was used in Western Blotting to examine the role of FAT4 in proliferation and metastasis, and its mechanisms of interaction in these processes.

Mon May 04 00:00:00 UTC 2020

In Vitro Effects of Vaspin on Porcine Granulosa Cell Proliferation, Cell Cycle Progression, and Apoptosis by Activation of GRP78 Receptor and Several Kinase Signaling Pathways Including MAP3/1, AKT, and STAT3.

International journal of molecular sciences

Kurowska P,Mlyczyńska E,Dawid M,Opydo-Chanek M,Dupont J,Rak A

PA5-22252 was used in Western Blotting to examine the effect of vaspin on granulosa (Gc) proliferation, cell cycle regulation, and apoptosis.

Tue Nov 19 00:00:00 UTC 2019

生物信息学

蛋白别名: 25 kDa caspase-9 dominant negative protein; APAF-3; apoptotic protease activating factor 3; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; cas9; CASP 9; CASP-9; caspase 9, apoptosis-related cysteine peptidase; Caspase-9; caspase-9 dominant negative form; Caspase-9 precursor; Caspase-9 subunit p10; Caspase-9 subunit p35; caspase-9-carboxyl-terminal divergent; CASPASE-9c; Caspase9; CTD; ICE-LAP6; ICE-like apoptotic protease 6; OTTHUMP00000002324; protein phosphatase 1, regulatory subunit 56; RNCASP9; RP11-265F14.3

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基因别名: APAF-3; APAF3; Casp-9-CTD; CASP9; Casp9_v1; ICE-LAP6; MCH6; PPP1R56

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UniProt ID: (Human) P55211

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Entrez Gene ID: (Human) 842, (Rat) 58918

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功能

cysteine-type endopeptidase activity protein binding enzyme activator activity peptidase activity SH3 domain binding protein kinase binding cysteine-type endopeptidase activity involved in apoptotic process cysteine-type peptidase activity

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