Invitrogen

Caspase 9 (Cleaved Asp315) Polyclonal Antibody

Caspase 9 (Cleaved Asp315) Antibody in Immunocytochemistry (ICC/IF)

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Caspase 9 (Cleaved Asp315) Antibody (PA5-17913) in ICC/IF

Immunofluorescence analysis of Caspase 9 (Cleaved Asp315) was performed using 70% confluent log phase HeLa cells treated with 1µM of Staurosporine for 3 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Caspase 9 (Cleaved Asp315) Rabbit Polyclonal Antib... View More

Caspase 9 (Cleaved Asp315) Antibody in Western Blot (WB)

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产品信息

PA5-17913

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看4篇已发表文章 4篇已发表文章

免疫细胞化学 (ICC/IF)

1:100

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免疫沉淀 (IP)

1:100

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues surrounding Asp315 of human caspase-9

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

6 µg/mL

纯化类型

Antigen affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10979038

产品详细信息

It is not recommended to aliquot this antibody.

This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.

靶标信息

Caspase 9 (ICE-like apoptotic protease 6, ICE LAP6, apoptotic protease Mch6, apoptotic protease activating factor 3, Apaf3) is a member of the peptidase family C14 that contains a CARD domain. Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspase 9 is active as a heterotetramer, is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Further, Caspase 9 is involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf- 1 leads to activation of the protease which then cleaves and activates caspase-3. Caspase 9 promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner, and proteolytically cleaves poly(ADP- ribose) polymerase (PARP). Caspase 9 is expressed in variety of human tissues. An isoform of rat Caspase-9 has been identified in which the C terminus of full-length Caspase-9 is replaced with an alternative peptide sequence. Mutations in Caspase 9 affect brain development. In mice, insufficient Caspase 9 have resulted in abnormal cerebrum development and neuron development. In humans, dysfunctional Caspase 9 expression vary from tissue to tissue. Low levels of Caspase 9 may play a role in cancer development and neurodegenerative diseases such as Alzheimer’s Disease (AD).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Simultaneous Inhibition of Mcl-1 and Bcl-2 Induces Synergistic Cell Death in Hepatocellular Carcinoma.

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Michalski M,Bauer M,Walz F,Tümen D,Heumann P,Stöckert P,Gunckel M,Kunst C,Kandulski A,Schmid S,Müller M,Gülow K

PA5-17913 was used in Western Blotting to indicate the potential of combining inhibitors of the BCL-2 family as new therapeutic options in HCC.

Thu Jun 08 00:00:00 UTC 2023

Simultaneous Inhibition of Mcl-1 and Bcl-2 Induces Synergistic Cell Death in Hepatocellular Carcinoma.

Biomedicines

Michalski M,Bauer M,Walz F,Tümen D,Heumann P,Stöckert P,Gunckel M,Kunst C,Kandulski A,Schmid S,Müller M,Gülow K

PA5-17913 was used in Western Blotting to indicate the potential of combining inhibitors of the BCL-2 family as new therapeutic options in HCC.

Thu Jun 08 00:00:00 UTC 2023

Purine nucleoside phosphorylase deficiency induces p53-mediated intrinsic apoptosis in human induced pluripotent stem cell-derived neurons.

Scientific reports

Tsui M,Biro J,Chan J,Min W,Dobbs K,Notarangelo LD,Grunebaum E

PA5-17913 was used in Immunocytochemistry-immunoflourescence to establish a potential mechanism for the neurological defects observed in PNP-deficient patients and reinforce the critical role that PNP has for neuronal viability.

Tue May 31 00:00:00 UTC 2022

Purine nucleoside phosphorylase deficiency induces p53-mediated intrinsic apoptosis in human induced pluripotent stem cell-derived neurons.

Scientific reports

Tsui M,Biro J,Chan J,Min W,Dobbs K,Notarangelo LD,Grunebaum E

Tue May 31 00:00:00 UTC 2022

miR‑302d‑3p regulates the viability, migration and apoptosis of breast cancer cells through regulating the TMBIM6‑mediated ERK signaling pathway.

Molecular medicine reports

Liao Y,Qiu Z,Bai L

PA5-17913 was used in Western Blot to identify the role of miR‚302D‚3p in breast cancer and its potential mechanism.

Wed Dec 01 00:00:00 UTC 2021

miR‑302d‑3p regulates the viability, migration and apoptosis of breast cancer cells through regulating the TMBIM6‑mediated ERK signaling pathway.

Molecular medicine reports

Liao Y,Qiu Z,Bai L

PA5-17913 was used in Western Blot to identify the role of miR‚302D‚3p in breast cancer and its potential mechanism.

Wed Dec 01 00:00:00 UTC 2021

Stingray Venom Proteins: Mechanisms of Action Revealed Using a Novel Network Pharmacology Approach.

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Kirchhoff KN,Billion A,Voolstra CR,Kremb S,Wilke T,Vilcinskas A

PA5-17913 was used in Immunocytochemistry to develop a novel network pharmacology approach based on multi-omics functional data integration to predict how stingray venom disrupts the physiological systems of target animals.

Fri Dec 24 00:00:00 UTC 2021

Expression Patterns of TNFα, MAdCAM1, and STAT3 in Intestinal and Skin Manifestations of Inflammatory Bowel Disease.

Journal of Crohn's & colitis

Vavricka SR,Galván JA,Dawson H,Soltermann A,Biedermann L,Scharl M,Schoepfer AM,Rogler G,Prinz Vavricka MB,Terracciano L,Navarini A,Zlobec I,Lugli A,Greuter T

PA5-17913 was used in Immunocytochemistry, Immunoprecipitation, Western Blot to investigate the role of TNFα and NFκB overexpression in skin biopsies from EN, PG, and psoriasis.

Wed Feb 28 00:00:00 UTC 2018

Inhibition of the prolyl isomerase Pin1 enhances the ability of sorafenib to induce cell death and inhibit tumor growth in hepatocellular carcinoma.

Oncotarget

Zheng M,Xu H,Liao XH,Chen CP,Zhang AL,Lu W,Wang L,Yang D,Wang J,Liu H,Zhou XZ,Lu KP

Published figure using Caspase 9 (Cleaved Asp315) polyclonal antibody (Product # PA5-17913) in Western Blot

Tue May 02 00:00:00 UTC 2017

Long Non-Coding RNA (lncRNA) Urothelial Carcinoma-Associated 1 (UCA1) Enhances Tamoxifen Resistance in Breast Cancer Cells via Inhibiting mTOR Signaling Pathway.

Medical science monitor : international medical journal of experimental and clinical research

Wu C,Luo J

PA517913 was used in immunocytochemistry to examine the role of UCA1 in tamoxifen resistance of estrogen receptor positive breast cancer cells

Fri Oct 21 00:00:00 UTC 2016

Human pluripotent stem cells have a novel mismatch repair-dependent damage response.

The Journal of biological chemistry

Lin B,Gupta D,Heinen CD

PA5-17913 was used in immunocytochemistry to explore DNA repair responses in human pluripotent stem cells

Fri Aug 29 00:00:00 UTC 2014

Human pluripotent stem cells have a novel mismatch repair-dependent damage response.

The Journal of biological chemistry

Lin B,Gupta D,Heinen CD

PA5-17913 was used in immunocytochemistry to explore DNA repair responses in human pluripotent stem cells

Fri Aug 29 00:00:00 UTC 2014

生物信息学

蛋白别名: APAF-3; apoptosis-related cysteine protease; apoptotic protease activating factor 3; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; cas9; CASP-9; caspase 9, apoptosis-related cysteine peptidase; Caspase-9; Caspase-9 subunit p10; Caspase-9 subunit p35; ICE-LAP6; ICE-like apoptotic protease 6; OTTHUMP00000002324; protein phosphatase 1, regulatory subunit 56

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基因别名: APAF-3; APAF3; CASP9; ICE-LAP6; MCH6; PPP1R56

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UniProt ID: (Human) P55211

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Entrez Gene ID: (Human) 842

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功能

cysteine-type endopeptidase activity protein binding enzyme activator activity peptidase activity SH3 domain binding protein kinase binding cysteine-type endopeptidase activity involved in apoptotic process

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